化学感觉系统允许细菌响应和适应环境条件。许多细菌含有不止一个化学感应系统,但是关于它们在调节不同功能中的具体作用的知识仍然很少。这里,我们通过分析模型植物病原体丁香假单胞菌pv的F6,F8和替代(非运动性)细胞功能(ACF)化学感应系统的功能来解决此问题。番茄。在这项工作中,我们将PsPto化学感受器分配给每个化学感应系统,我们首次使用低温电子层析成像技术可视化了PsPto的F6和F8化学感应系统。我们确认趋化性和游泳运动受F6系统控制,我们演示了F8和ACF系统中的不同组件如何调节游泳运动。我们还确定了来自F6和F8化学感应系统的激酶和反应调节剂如何在生物膜的调节中不协同工作,而来自ACF系统的两种组分共同调节这些性状。此外,我们展示了F6,F8和ACF激酶如何与ACF反应调节因子WspR相互作用,支持化学感应系统之间的串扰。最后,我们揭示了所有化学感应系统如何在调节毒力中发挥作用。
目的:通过化学感应系统进行化学感知是细菌生存的基本特征,因为它允许细菌与周围环境相互作用。在植物病原体的情况下,进入宿主并达到完全毒力是特别相关的。多种化学感应系统使细菌在对外部信号的反应中表现出更广泛的可塑性。这里,我们对模型植物病原体丁香假单胞菌pv中的F6,F8和替代(非运动性)细胞功能化学感应系统进行了深入表征。番茄DC3000.这些化学感应系统调节关键的毒力相关性状,像运动性和生物膜的形成。此外,我们揭示了在激酶和反应调节剂之间的相互作用水平上,这些化学感应系统之间的意外串扰。这项工作显示了新颖的结果,有助于了解化学感应系统及其在替代趋化性功能中的作用。
Chemosensory systems allow bacteria to respond and adapt to environmental conditions. Many bacteria contain more than one chemosensory system, but knowledge of their specific roles in regulating different functions remains scarce. Here, we address this issue by analyzing the function of the F6, F8, and alternative (non-motility) cellular functions (ACF) chemosensory systems of the model plant pathogen Pseudomonas syringae pv. tomato. In this work, we assign PsPto chemoreceptors to each chemosensory system, and we visualize for the first time the F6 and F8 chemosensory systems of PsPto using cryo-electron tomography. We confirm that chemotaxis and swimming motility are controlled by the F6 system, and we demonstrate how different components from the F8 and ACF systems also modulate swimming motility. We also determine how the kinase and response regulators from the F6 and F8 chemosensory systems do not work together in the regulation of biofilm, whereas both components from the ACF system contribute together to regulate these traits. Furthermore, we show how the F6, F8, and ACF kinases interact with the ACF response regulator WspR, supporting crosstalk among chemosensory systems. Finally, we reveal how all chemosensory systems play a role in regulating virulence.
OBJECTIVE: Chemoperception through chemosensory systems is an essential feature for bacterial survival, as it allows bacterial interaction with its surrounding environment. In the case of plant pathogens, it is especially relevant to enter the host and achieve full virulence. Multiple chemosensory systems allow bacteria to display a wider plasticity in their response to external signals. Here, we perform a deep characterization of the F6, F8, and alternative (non-motility) cellular functions chemosensory systems in the model plant pathogen Pseudomonas syringae pv. tomato DC3000. These chemosensory systems regulate key virulence-related traits, like motility and biofilm formation. Furthermore, we unveil an unexpected crosstalk among these chemosensory systems at the level of the interaction between kinases and response regulators. This work shows novel results that contribute to the knowledge of chemosensory systems and their role in functions alternative to chemotaxis.