PDF(Pubmed)
Abstract：
Cherry tomatoes, a very popular fruit, are highly susceptible to microbial infestation, which cause significant economic losses. In order to preserve cherry tomatoes better, we treat them with a Chitosan (CTS) and Curdlan (CUR) composite coating. The lowest inhibitory concentration of CTS/CUR composite coating on Serratia marcescens and Pseudomonas syringae, the growth curves, and the changes of the cell lysis rate were determined to explore the inhibitory mechanism of CTS/CUR composite coating on Serratia marcescens and Pseudomonas syringae and the microscopic morphology of Serratia marcescens and Pseudomonas syringae was observed using scanning electron microscopy at the same time. The results showed that the CTS/CUR composite coating could effectively inhibit the growth of Serratia marcescens and Pseudomonas, and the inhibitory effect reflected the concentration-dependent characteristics. The electron microscopy results indicated that the inhibition of Serratia marcescens and Pseudomonas syringae by the CTS/CUR composite coating might originate from its disruptive effect on the cell wall and cell membrane of the bacterium.
摘要：
樱桃番茄,一种非常受欢迎的水果，极易受到微生物侵扰，造成重大经济损失。为了更好地保存樱桃番茄，我们用壳聚糖（CTS）和Curdlan（CUR）复合涂层处理它们。CTS/CUR复合涂层对粘质沙雷氏菌和丁香假单胞菌的最低抑菌浓度,生长曲线，并测定细胞裂解率的变化，以探讨CTS/CUR复合涂层对粘质沙雷氏菌和丁香假单胞菌的抑制机制，同时用扫描电镜观察粘质沙雷氏菌和丁香假单胞菌的微观形态。结果表明,CTS/CUR复合涂层能有效抑制粘质沙雷氏菌和假单胞菌的生长,抑制作用反映了浓度依赖性特征。电子显微镜结果表明，CTS/CUR复合涂层对粘质沙雷氏菌和丁香假单胞菌的抑制作用可能源于其对细菌细胞壁和细胞膜的破坏作用。
