Abstract：
One of the major plant stress level indicators is reactive oxygen species (ROS). They have been known to play a central role in regulating plant responses to various environmental stresses. This book chapter specifically covers abiotic stress induced by a drought hormone abscisic acid and biotic stress induced by Pseudomonas syringe DC3000 on single cell-type guard cells. We describe in detail the measurement of ROS production starting from sample preparation to data analysis by fluorescence intensity acquisition using ImageJ software. We discussed the problems faced while performing the experiment and addressed how to overcome them by providing specific guidelines to ensure high quality repeatable data.
摘要：
主要的植物胁迫水平指标之一是活性氧(ROS)。已知它们在调节植物对各种环境胁迫的反应中起着核心作用。本书章节特别涵盖了干旱激素脱落酸诱导的非生物胁迫和假单胞菌注射器DC3000在单细胞型保卫细胞上诱导的生物胁迫。我们详细描述了从样品制备到使用ImageJ软件通过荧光强度采集进行数据分析的ROS产生的测量。我们讨论了在执行实验时面临的问题，并通过提供特定的指南以确保高质量的可重复数据来解决这些问题。
