目的:我们先前显示了L-赖氨酸(Lys)的有益作用,一个化学伴侣,减轻糖尿病大鼠和2型糖尿病患者的糖尿病并发症。在这里,我们评估了Lys与维生素C和锌(Lys+VC+Zn)联合给药的效果,在糖尿病大鼠中。
方法:将链脲佐菌素(50mg/Kg)注射到雄性成年Wistar大鼠中,以诱发糖尿病。然后,正常和糖尿病大鼠分别用Lys和LysVCZn治疗5个月。所以,两组的饮用水中均有0.1%的Lys。对照组仅接受水。在实验过程中,体重,并在血液中确定了各种参数,血清/血浆,和老鼠的尿液。
结果:生化指标的测定证实了大鼠糖尿病的诱发及其并发症。Lys或Lys+VC+Zn治疗导致血糖和蛋白质糖基化降低(AGEs和HbA1c降低),胰岛素分泌增加,减轻胰岛素抵抗和HOMA-IR,改善血脂和HDL功能(LCAT和PON1),增强的抗氧化剂状态(FRAP和AOPP),改善肾功能(减少微量白蛋白尿,血清尿素,和肌酐),和增加的伴侣能力(HSP70)。LysVCZn对这些参数的影响比单独的Lys更好。
结论:这项研究的结果表明,Lys的共同给药,一个化学伴侣,在糖尿病大鼠模型中,添加两种抗氧化剂(VC和Zn)可增强其抗糖尿病作用并预防糖尿病并发症。
OBJECTIVE: We previously showed the beneficial effect of L-Lysine (Lys), a chemical chaperone, on reducing diabetic complications in diabetic rats and type 2 diabetic patients. Herein, we evaluated the effect of Lys co-administration with Vitamin C and Zinc (Lys+VC+Zn), in diabetic rats.
METHODS: The streptozotocin (50 mg/Kg) was injected into male adult Wistar rats to induce diabetes. Then, different groups of normal and diabetic rats were treated with Lys and Lys+VC+Zn for five months. So, there were 0.1 % Lys in the drinking water of both groups. The control groups received water alone. During the experiment, the body weight, and various parameters were determined in the blood, serum/plasma, and urine of the rats.
RESULTS: The determination of biochemical indexes confirmed diabetes induction and its complications in rats. Treatment with either Lys or Lys+VC+Zn resulted in reduced blood glucose and protein glycation (decreasing AGEs and HbA1c), increased insulin secretion, alleviated insulin resistance and HOMA-IR, improved lipid profile and HDL functionality (LCAT and PON1), enhanced antioxidant status (FRAP and AOPP), improved kidney function (decreased microalbuminuria, serum urea, and creatinine), and increased chaperone capacity (HSP70). Lys+VC+Zn showed better effects on these parameters than Lys alone.
CONCLUSIONS: The results of this study indicated that co-administration of Lys, a chemical chaperone, with two antioxidants (VC and Zn) potentiates its antidiabetic effects and prevent diabetic complications in rat model of diabetes.