PDF(Pubmed)
Abstract：
The enhancement of seed germination by using nanoparticles (NPs) holds the potential to elicit the synthesis of more desired compounds with important biomedical applications, such as preventing protein glycation, which occurs in diabetes. Here, we used 7 nm and 100 nm ZnO and 4.5 nm and 16.7 nm Fe2O3 NPs to treat sunflower seeds. We evaluated the effects on germination, total phenolic content, and the anti-glycation potential of extracted polyphenols. Sunflower seeds were allowed to germinate in vitro after soaking in NP solutions of different concentrations. Polyphenols were extracted, dosed, and used in serum albumin glycation experiments. The germination speed of seeds was significantly increased by the 100 nm ZnO NPs and significantly decreased by the 4.5 nm Fe2O3 NPs. The total phenolic content (TPC) of seeds was influenced by the type of NP, as ZnO NPs enhanced TPC, and the size of the NPs, as smaller NPs led to improved parameters. The polyphenols extracted from seeds inhibited protein glycation, especially those extracted from seeds treated with 7 nm ZnO. The usage of NPs impacted the germination speed and total polyphenol content of sunflower seeds, highlighting the importance of NP type and size in the germination process.
摘要：
通过使用纳米颗粒（NPs）增强种子萌发具有引发具有重要生物医学应用的更多所需化合物的合成的潜力。例如防止蛋白质糖基化，发生在糖尿病中。这里,我们使用7nm和100nm的ZnO和4.5nm和16.7nm的Fe2O3NP处理向日葵种子。我们评估了对发芽的影响，总酚含量，和提取的多酚的抗糖基化潜力。在不同浓度的NP溶液中浸泡后，使向日葵种子在体外发芽。多酚被提取，剂量,用于血清白蛋白糖化实验。100nm的ZnONPs显着提高了种子的发芽速度，而4.5nm的Fe2O3NPs显着降低了种子的发芽速度。种子总酚含量(TPC)受NP类型的影响,随着ZnONPs增强TPC，和NPs的大小，较小的NP导致参数的改善。从种子中提取的多酚抑制蛋白质糖基化，特别是从用7nmZnO处理的种子中提取的。NPs的使用影响向日葵种子的发芽速度和总多酚含量，强调NP类型和大小在发芽过程中的重要性。
