Abstract：
Nonenzymatic glycation of proteins is accelerated in the context of elevated blood sugar levels in diabetes. Vitamin and mineral deficiencies are strongly linked to the onset and progression of diabetes. The antiglycation ability of various water- and fat-soluble vitamins, along with trace minerals like molybdenum (Mo), manganese (Mn), magnesium (Mg), chromium, etc., have been screened using Bovine Serum Albumin (BSA) as in vitro model. BSA was incubated with methylglyoxal (MGO) at 37 °C for 48 h, along with minerals and vitamins separately, along with controls and aminoguanidine (AG) as a standard to compare the efficacy of the minerals and vitamins. Further, their effects on renal cells\' (HEK-293) antioxidant potential were examined. Antiglycation potential is measured by monitoring protein glycation markers, structural and functional modifications. Some minerals, Mo, Mn, and Mg, demonstrated comparable inhibition of protein-bound carbonyl content and ß-amyloid aggregation at maximal physiological concentrations. Mo and Mg protected the thiol group and free amino acids and preserved the antioxidant potential. Vitamin E, D, B1 and B3 revealed significant glycation inhibition and improved antioxidant potential in HEK-293 cells as assessed by estimating lipid peroxidation, SOD and glyoxalase activity. These results emphasize the glycation inhibitory potential of vitamins and minerals, indicating the use of these micronutrients in the prospect of the therapeutic outlook for diabetes management.
摘要：
在糖尿病血糖水平升高的情况下，蛋白质的非酶糖基化加速。维生素和矿物质缺乏与糖尿病的发病和进展密切相关。各种水溶性和脂溶性维生素的抗糖基化能力，以及钼（Mo）等微量矿物质，锰(Mn),镁(Mg),铬,等。,已经使用牛血清白蛋白(BSA)作为体外模型进行了筛选。BSA与甲基乙二醛（MGO）在37°C下孵育48小时，与矿物质和维生素分开，以及对照和氨基胍（AG）作为比较矿物质和维生素功效的标准。Further,检查了它们对肾细胞（HEK-293）抗氧化潜力的影响。通过监测蛋白质糖基化标志物来测量抗糖基化潜力，结构和功能修改。一些矿物质,Mo,Mn,Mg,在最大生理浓度下，对蛋白质结合的羰基含量和β-淀粉样蛋白聚集的抑制作用相当。Mo和Mg保护了巯基和游离氨基酸，并保留了抗氧化潜力。维生素E,D,B1和B3显示显著的糖化抑制和改善的抗氧化潜力在HEK-293细胞通过评估脂质过氧化评估，SOD和乙二醛酶活性。这些结果强调了维生素和矿物质的糖化抑制潜力，表明这些微量营养素在糖尿病管理治疗前景中的应用前景。
