Photon-initiated photoacoustic streaming (PIPS) with an Er: YAG laser has been introduced in root canal treatment to improve irrigation and facilitate the removal of bacteria in the root canal system. This study aimed to compare the antibacterial effectiveness of two different root canal irrigation techniques, conventional needle irrigation (CNI) and PIPS, using 1% sodium hypochlorite (NaOCl), in the treatment of teeth with apical periodontitis. Sixty patients with a total of sixty teeth affected by apical periodontitis were included in this study. The teeth underwent root canal therapy, and after mechanical instrumentation, they were randomly assigned to two groups (n = 30) based on the final irrigation protocol: CNI or PIPS with 1% NaOCl. Bacterial suspensions in the root canals were evaluated using Adenosine 5\'-triphosphate (ATP) assay kit after mechanical instrumentation and after final irrigation. Then, a follow-up was conducted after 7 days. The results revealed that final irrigation significantly reduced ATP values in both the CNI and PIPS groups (P < 0.001). The ATP values after final irrigation was greater in the CNI group compared to the PIPS group (P < 0.001). After a 7-day follow-up, percussion tenderness and fistula were significantly resolved in both groups (P < 0.05). A multivariate linear regression model was used to identify the factors that influence post irrigation ATP values. The analysis demonstrated that pre-operative percussion tenderness (P = 0.006), the presence of a fistula (P < 0.001) and the method used in the final irrigation (P < 0.001) had a significant impact on the ATP value after final irrigation. These results indicate that employing PIPS with 1% NaOCl as the final irrigation protocol exhibited superior antibacterial effectiveness and has the potential to enhance clinical outcomes in the treatment of teeth afflicted with apical periodontitis.

UNASSIGNED: Photon-induced photoacoustic streaming (PIPS) with pineapple peel extract (PPE) and ethylenediaminetetraacetic acid (EDTA) as a final endodontic irrigant on the push-out bond strength (PBS) of root filling material to conventional irrigation NaOCl with EDTA.
UNASSIGNED: An in vitro study at Dar Al Uloom University was conducted over three months. Root canal preparation was performed. Based on final irrigation, all the specimens were divided randomly into four groups (n=10) Group 1: 2.25% NaOCl+ 17% EDTA (control), Group-2: 2.25% NaOCl+PIPS + 6.25% PPE, Group-3: 2.25% NaOCl+PIPS + 17% EDTA, Group-4: 2.25% NaOCl + 6.25% PPE. Canals were obturated and sealed with AH Plus sealer. Root sectioning was performed at 1mm thickness and PBS testing was performed using the universal testing machine. The debonded samples were analyzed for failure mode. ANOVA compared the means and standard deviations (SD) of all investigated group. Assessment of multiple comparisons was performed using Tukey\'s post hoc test.
UNASSIGNED: The maximum PBS was demonstrated by the coronal section of Group-2 (2.25%NaOCl +PIPS+6.25%PPE) specimens (8.21±0.81MPa). The apical section of Group-1 (2.25% NaOCl+17% EDTA) specimens demonstrated minimum bond strength (2.80±0.18 MPa). The intergroup comparison revealed that Group-3 (2.25% NaOCl +PIPS +17% EDTA) and Group-2 demonstrated comparable outcomes (p>0.05). Group-4 (2.25% NaOCl +6.25% PPE) specimens established significantly lower values than Group-3 and Group-4 (p<0.05).
UNASSIGNED: Photon-induced photoacoustic streaming with pineapple peel extract and EDTA demonstrated better bond strength of root canal sealer and have the potential to be used as the final irrigant.

Objective: To evaluate the outcome of post space disinfection using Er,Cr:YSGG laser (ECYL), riboflavin photosensitizer (RFP), and photon-induced photoacoustic streaming (PIPS) along with ethylene diamine tetra acetic acid (EDTA) as final irrigant on the bond values of glass fiber post (GFP) to the canal wall. Materials and methods: Forty human mandibular premolars were subjected to root canal treatment after decoronation till the cementoenamel junction. Post space was prepared using a Gates-Glidden drill leaving 4 mm of gutta perch at the apical third of the root section. Random allocation of samples was performed into four groups based on the post space disinfection (n = 10 each): Group 1: 1% RFP and 17% EDTA, group 2: 5.25% sodium hypochlorite (NaOCl) and 17% EDTA (control), group 3: PIPS and 17% EDTA, and group 4: ECYL and 17% EDTA. GFP was luted using Rely X Unicem. A universal testing machine and stereomicroscope were used to assess the push-out bond strength (PBS) and failure mode, respectively. Analysis of variance determined the PBS amid different experimental groups at three root levels. Multiple group comparison using means of tested groups was identified using post hoc Tukey. Results: The coronal third of group 2 (5.25% NaOCl +17% EDTA) demonstrated the highest PBS (8.83 ± 0.11 MPa). However, the lowest bond integrity (4.15 ± 0.52 MPa) was displayed in an apical third of group 1 (RFP +17% EDTA). The intergroup comparison revealed that 5.25% NaOCl +17% EDTA at all three levels demonstrated comparable outcomes of PBS with PIPS +17% EDTA and ECYL +17% EDTA specimens (p > 0.05). However, riboflavin +17% EDTA demonstrated the lowest bond strength (p < 0.05). Conclusions: Er,Cr: YSGG and PIPS in combination with 17% EDTA demonstrated comparable outcomes of bond score with those of conventional gold standard irrigation regime 5.25% NaOCl.

Objective: This study aimed to evaluate the effectiveness of photon-induced photoacoustic streaming (PIPS), XP-endo Finisher (XPF), and passive ultrasonic irrigation (PUI) for the removal of root canal filling materials from oval root canals. Background: Various adjunctive irrigation techniques following mechanical preparation have been applied to enhance removal of fillings during root canal retreatment. However, whether there is superiority of one approach over others remains controversial. Methods: Thirty extracted single-rooted teeth with oval-shaped canals were instrumented with the ProTaper Next (PTN) system and obturated with the warm vertical compaction technique. After storage at 37°C for 1 month, retreatment was performed with the PTN system up to size X4. The teeth were then randomly assigned into three groups (n = 10) that were, respectively, subjected to supplementary irrigation with different protocols: PIPS, PUI, and XPF, followed by measurement of filling material volumes using high-resolution micro-computed tomography. Results: The PTN preparation resulted in significant reductions in residual filling materials (p < 0.05), whereas PIPS, PUI, and XPF moderately improved the removal of the remnants (p > 0.05). The residual filling materials after supplementary irrigation ranged from 0.384 ± 0.181 to 0.421 ± 0.143 mm3 in oval canals. There was no significant difference in the effectiveness among the PIPS, PUI, and XPF approaches (p > 0.05). Conclusions: Mechanical preparations are useful for removing most root fillings during retreatment in oval-shaped canals. PIPS can reduce residual root-filling materials to the similar extent as PUI and XPF.

OBJECTIVE: The aim of this study is to measure and compare the amount of apical extrusion of the methylene blue as photosensitizer using Laser-activated irrigation(LAI) techniques (SWEEPS, PIPS) and photodynamic therapy (PDT).
METHODS: 40 single-rooted premolar teeth were selected and their root canals were cleaned and prepared. The photosensitizer, Methylene blue (MB), was used as dye which applied inside the root canals and the access cavities. Then the teeth were randomly allocated to 4 groups. Different techniques (SWEEPS, PIPS, PDT) were used in 3 groups. One group received no activation. Data related to the concentration of the extruded methylene blue was measured using ultraviolet and visible (UV-Vis) absorption spectroscopy. Absorption peak intensity of MB in the wavelength of 668 nm was considered as the concentration index according to Beer Lambert law. Taking into account the normal distribution of this data, one-way ANOVA analysis was used to evaluate the effects of the independent variables on the amount of apical extrusion of the irrigant.
RESULTS: Based on the results of ANOVA analysis, there was no significant difference between the amounts of apical extrusion of MB between different groups (p = 0.628) CONCLUSION: Using SWEEPS and PIPS techniques, despite diffusion of the photosensitizer, didn\'t lead to any difference in the amount of apical extrusion. Therefore, these LAI techniques can be used safely for root canal irrigation.

OBJECTIVE: The aim of the present study was to visualize and compare the cavitation effect and fluid dynamics induced by photon-induced photoacoustic streaming (PIPS) using sodium hypochlorite (NaOCl) with different concentrations as irrigant.
METHODS: Forty artificial root canals were prepared using MTWO Niti file up to size #25/.06. The canals were randomly divided into four groups (n = 10/group). High-speed camera was used to visualize and compare the cavitation effect induced by PIPS in the artificial root canals containing saline or NaOCl. Fluid velocity and Reynolds number of saline, 1%-, 2.5%- and 5.25% NaOCl irrigants induced by PIPS in the apical region were calculated using TEMA 2D software while the fluid motions were recorded.
RESULTS: Visualization profile revealed that NaOCl presented a stronger cavitation effect and fluid dynamics than saline during PIPS activation. In the apical region, 1% NaOCl group presented the highest average velocity of 3.868 m/s, followed by 2.5% NaOCl group (3.685 m/s), 5.25% NaOCl group (2.353 m/s) and saline group (1.268 m/s), corresponding to Reynolds number of 1653.173, 1572.196, 995.503 and 477.692. Statistically higher fluid velocity was calculated in 1% and 2.5% NaOCl groups compared to saline group, respectively (p < 0.05).
CONCLUSIONS: The application of NaOCl and its concentration significantly influence the cavitation effect and fluid dynamics during PIPS activation. 1% and 2.5% NaOCl groups presented a more violent fluid motion in the apical region when activated by PIPS.

In recent years, the concept of minimally invasive endodontics has been proposed, which could be described as preventing or treating diseases by preserving more dental tissue and creating minimal damage. In the process of root canal preparation, it was recommended to use instruments with a smaller taper to preserve more tooth tissue and improve the preservation rate of the affected teeth. Photon-induced photoacoustic streaming (PIPS) was a new type of laser-activated irrigation technology, which was now widely used in endodontic treatment. The purpose of this article was to evaluate the bactericidal effect of PIPS with NaOCl in root canals with different widths or tapers.
Twenty-three maxillary first molars with three independent root canals were included in this study. The mesiobuccal (MB), distobuccal (DB), and palatal (P) root canals were prepared at sizes of #10/.02, #25/.02, and #25/.06, respectively. After being incubated with a bacterial suspension for 4 weeks, the specimen were irrigated with 2% NaOCl activated by conventional needle irrigation (CNI) (n = 10) or PIPS (n = 10). Three specimen were not treated (control group). Before and after irrigation, the presence of bacteria was assessed with an adenosine 5\'-triphosphate (ATP) assay kit and biofilms were assessed using confocal laser scanning microscopy and scanning electron microscopy.
In specimen prepared using PIPS irrigation, the ATP was reduced by more than 98%. When the root canal taper was 0.02, the size #25 root canals had a higher percentage of dead bacteria than the size #10 root canals in all regions (P < 0.05) in the PIPS group. When the root canal width was #25, the 0.02 taper group had a higher percentage of dead bacteria than the 0.06 taper group in the apical region (P < 0.05), except coronal and middle regions (P > 0.05). PIPS irrigation results in a greater percentage of dead bacteria and reduction of ATP in size #10/.02 root canals than CNI in size #25/.06 root canals in three regions (P < 0.05).
Increasing the width from #10 to #25 improves the bactericidal effect of PIPS in the root canal. Increasing the taper of the root canal from 0.02 to 0.06 at size #25 did not affect the bactericidal effects of PIPS. PIPS resulted in more dead bacteria in specimen with smaller tapers and root canal widths than CNI. PIPS can be used to clean the smear layer in the coronal region and open the dentin tubules.
Activation of irrigants with PIPS brought about significant bacterial reduction smaller tapers and width root canals compared to CNI, which was beneficial to prevent excessive loss of tooth tissue and conserve the structural integrity of teeth.

OBJECTIVE: This study aimed to compare the efficacy of silver nanoparticles (AgNPs) irrigating solution alone and following activation with photon-induced photoacoustic streaming (PIPS), photodynamic therapy (PDT) with indocyanine green (ICG), passive ultrasonic irrigation (PUI), and manual dynamic activation (MDA) method for elimination of Enterococcus faecalis (E. faecalis) from the root canal system.
METHODS: A total of 59 extracted human single-rooted teeth were collected and prepared. E. faecalis was inoculated into the root canals and incubated for 4 weeks. The teeth were then randomly divided into five experimental groups (n = 10): the AN group, irrigation with AgNPs alone; the AN/ICG/DL group, irrigation with AgNPs and ICG, then activation with diode laser; the AN/PIPS group, irrigation with AgNPs and activation with 0.3 W Er: YAG laser; the AN/MDA group, irrigation with AgNPs and activation with tapered gutta-percha; and the AN/PUI group, irrigation with AgNPs and activation with ultrasonic. Also, two control groups of irrigation with 2.5% sodium hypochlorite (n = 5) and no intervention (n = 4) were also used. Samples were collected from the dentinal chips before and after the intervention, and the percentage of reduction in colony count was calculated.
RESULTS: A significant reduction in E. faecalis colony count was noted in all groups (P < 0.05). Maximum reduction in colony count was noted in AN/PIPS and AN/PUI groups by 91.03 and 91.29%, respectively. Minimum reduction was noted in the AN group alone.
CONCLUSIONS: Activation with PUI and PIPS enhanced the efficacy of AgNPs irrigating solution for elimination of E. faecalis from the root canal system.
CONCLUSIONS: AgNPs activated by ultrasound or PIPS can be used as an adjunct for disinfection of the root canal system in endodontic treatment.

This study aimed to evaluate the effect of optimized irrigation with photon-induced photoacoustic streaming (PIPS) activation of different irrigants (distilled water or ethylenediaminetetraacetic acid [EDTA]) on smear layer removal, dentin microhardness, attachment morphology, and survival of stem cells of the apical papilla (SCAP) in an organotypic root canal model.
A total of 144 standardized root segments were randomly allocated into 6 groups for irrigation: (i) NaOCl group, (ii) NaOCl + EDTA group, (iii) NaOCl + PIPS (distilled water) group, (iv) NaOCl + PIPS (EDTA) group, (v) NaOCl + EDTA + PIPS (distilled water) group, and (vi) NaOCl + EDTA + PIPS (EDTA) group. Each group was divided into four subgroups for assessment: (i) dentin cleanliness; (ii) dentin microhardness; (iii) cell attachment morphology; and (iv) viable SCAP quantification.
Compared with the control groups, the NaOCl + EDTA + PIPS (EDTA) group showed higher efficiency in smear layer removal and in increasing SCAP viability with more stretched cellular morphology. There were no statistically significant differences in either smear layer removal effect, dentin microhardness, attachment morphology, or survival of SCAP among the other groups when optimized with PIPS (distilled water or EDTA) (P > 0.05).
Our findings indicated that irrigation optimized with PIPS activation of EDTA for 40 seconds was conducive to smear layer removal without additional dentin microhardness decrease. Additionally, this irrigation created more cell-friendly dentin conditioning than other approaches, which was beneficial for the attachment and survival of SCAP. Lasers Surg. Med. © 2021 Wiley Periodicals LLC.

Background and objective: The aim of this study is to compare the bleaching effect of photon-induced photoacoustic streaming (PIPS) using carbamide peroxide or sodium perborate-20% hydrogen peroxide (H2O2) compared with walking-bleaching techniques using sodium perborate-20% H2O2 or carbamide peroxide without PIPS on artificially colored teeth. Materials and methods: Root canal treatments of 85 extracted human maxillary central incisors were completed and artificially stained using sheep\'s blood. Teeth were randomly grouped into five groups according to the bleaching techniques to be tested: Group 1: walking bleaching with carbamide peroxide; Group 2: walking bleaching with sodium perborate-20% H2O2; Group 3: PIPS+carbamide peroxide; Group 4: PIPS+sodium perborate-20% H2O2; and Group 5: control. Experimental specimens were bleached for 3 weeks. Buccal surfaces of the crowns were used for spectrophotometric measurements, limited with a 7-mm external diameter rounded strip, before bleaching and on days 7, 14, and 21. The total change in color (ΔE*) were analyzed with Welch\'s ANOVA at a significance level of p = 0.05 and Tamhane post-hoc test was used for multiple comparisons. Results: Starting from the second week, Group 4 showed significantly better bleaching effect compared with the other groups (p < 0.05). The other experimental groups also have bleaching effects, but there was no statistically significant difference between Group 1, Group 2, and Group 3 (p > 0.05). Conclusions: Sodium perborate-20% H2O2 activated with PIPS gave a bleaching effect superior to that of walking-bleaching techniques or PIPS using with carbamide peroxide.