PDF(Sci-hub)
Abstract：
This study aimed to evaluate the effect of optimized irrigation with photon-induced photoacoustic streaming (PIPS) activation of different irrigants (distilled water or ethylenediaminetetraacetic acid [EDTA]) on smear layer removal, dentin microhardness, attachment morphology, and survival of stem cells of the apical papilla (SCAP) in an organotypic root canal model.
A total of 144 standardized root segments were randomly allocated into 6 groups for irrigation: (i) NaOCl group, (ii) NaOCl + EDTA group, (iii) NaOCl + PIPS (distilled water) group, (iv) NaOCl + PIPS (EDTA) group, (v) NaOCl + EDTA + PIPS (distilled water) group, and (vi) NaOCl + EDTA + PIPS (EDTA) group. Each group was divided into four subgroups for assessment: (i) dentin cleanliness; (ii) dentin microhardness; (iii) cell attachment morphology; and (iv) viable SCAP quantification.
Compared with the control groups, the NaOCl + EDTA + PIPS (EDTA) group showed higher efficiency in smear layer removal and in increasing SCAP viability with more stretched cellular morphology. There were no statistically significant differences in either smear layer removal effect, dentin microhardness, attachment morphology, or survival of SCAP among the other groups when optimized with PIPS (distilled water or EDTA) (P > 0.05).
Our findings indicated that irrigation optimized with PIPS activation of EDTA for 40 seconds was conducive to smear layer removal without additional dentin microhardness decrease. Additionally, this irrigation created more cell-friendly dentin conditioning than other approaches, which was beneficial for the attachment and survival of SCAP. Lasers Surg. Med. © 2021 Wiley Periodicals LLC.
A total of 144 standardized root segments were randomly allocated into 6 groups for irrigation: (i) NaOCl group, (ii) NaOCl + EDTA group, (iii) NaOCl + PIPS (distilled water) group, (iv) NaOCl + PIPS (EDTA) group, (v) NaOCl + EDTA + PIPS (distilled water) group, and (vi) NaOCl + EDTA + PIPS (EDTA) group. Each group was divided into four subgroups for assessment: (i) dentin cleanliness; (ii) dentin microhardness; (iii) cell attachment morphology; and (iv) viable SCAP quantification.
Compared with the control groups, the NaOCl + EDTA + PIPS (EDTA) group showed higher efficiency in smear layer removal and in increasing SCAP viability with more stretched cellular morphology. There were no statistically significant differences in either smear layer removal effect, dentin microhardness, attachment morphology, or survival of SCAP among the other groups when optimized with PIPS (distilled water or EDTA) (P > 0.05).
Our findings indicated that irrigation optimized with PIPS activation of EDTA for 40 seconds was conducive to smear layer removal without additional dentin microhardness decrease. Additionally, this irrigation created more cell-friendly dentin conditioning than other approaches, which was beneficial for the attachment and survival of SCAP. Lasers Surg. Med. © 2021 Wiley Periodicals LLC.
摘要：
本研究旨在评估不同灌溉剂（蒸馏水或乙二胺四乙酸[EDTA]）的光子诱导光声流（PIPS）活化优化灌溉对涂抹层去除的影响，牙本质显微硬度，附着形态，和根尖乳头干细胞（SCAP）在器官型根管模型中的存活。
总共144个标准化的根段被随机分为6组进行灌溉：（i）NaOCl组，(ii)NaOCl+EDTA基团，(iii)NaOCl+PIPS(蒸馏水)组,(iv)NaOCl+PIPS(EDTA)基团，(v)NaOCl+EDTA+PIPS(蒸馏水)组,和(vi)NaOCl+EDTA+PIPS(EDTA)基团。每组分为四个亚组进行评估：（i）牙本质清洁度；（ii）牙本质显微硬度；（iii）细胞附着形态；（iv）可行的SCAP定量。
与对照组相比，NaOClEDTAPIPS（EDTA）组在去除涂片层和增加SCAP活力方面显示出更高的效率，并且细胞形态更加伸展。两种涂片层去除效果均无统计学差异，牙本质显微硬度，附着形态，用PIPS（蒸馏水或EDTA）优化时，SCAP在其他组之间的存活率（P>0.05）。
我们的发现表明，用EDTA的PIPS活化40秒优化的灌溉有利于去除涂抹层，而不会降低额外的牙本质显微硬度。此外,这种灌溉比其他方法创造了更多的细胞友好的牙本质调理，有利于SCAP的附着和存活。激光器Surg.Med.©2021威利期刊有限责任公司。
总共144个标准化的根段被随机分为6组进行灌溉：（i）NaOCl组，(ii)NaOCl+EDTA基团，(iii)NaOCl+PIPS(蒸馏水)组,(iv)NaOCl+PIPS(EDTA)基团，(v)NaOCl+EDTA+PIPS(蒸馏水)组,和(vi)NaOCl+EDTA+PIPS(EDTA)基团。每组分为四个亚组进行评估：（i）牙本质清洁度；（ii）牙本质显微硬度；（iii）细胞附着形态；（iv）可行的SCAP定量。
与对照组相比，NaOClEDTAPIPS（EDTA）组在去除涂片层和增加SCAP活力方面显示出更高的效率，并且细胞形态更加伸展。两种涂片层去除效果均无统计学差异，牙本质显微硬度，附着形态，用PIPS（蒸馏水或EDTA）优化时，SCAP在其他组之间的存活率（P>0.05）。
我们的发现表明，用EDTA的PIPS活化40秒优化的灌溉有利于去除涂抹层，而不会降低额外的牙本质显微硬度。此外,这种灌溉比其他方法创造了更多的细胞友好的牙本质调理，有利于SCAP的附着和存活。激光器Surg.Med.©2021威利期刊有限责任公司。
