METHODS: A total of 59 extracted human single-rooted teeth were collected and prepared. E. faecalis was inoculated into the root canals and incubated for 4 weeks. The teeth were then randomly divided into five experimental groups (n = 10): the AN group, irrigation with AgNPs alone; the AN/ICG/DL group, irrigation with AgNPs and ICG, then activation with diode laser; the AN/PIPS group, irrigation with AgNPs and activation with 0.3 W Er: YAG laser; the AN/MDA group, irrigation with AgNPs and activation with tapered gutta-percha; and the AN/PUI group, irrigation with AgNPs and activation with ultrasonic. Also, two control groups of irrigation with 2.5% sodium hypochlorite (n = 5) and no intervention (n = 4) were also used. Samples were collected from the dentinal chips before and after the intervention, and the percentage of reduction in colony count was calculated.
RESULTS: A significant reduction in E. faecalis colony count was noted in all groups (P < 0.05). Maximum reduction in colony count was noted in AN/PIPS and AN/PUI groups by 91.03 and 91.29%, respectively. Minimum reduction was noted in the AN group alone.
CONCLUSIONS: Activation with PUI and PIPS enhanced the efficacy of AgNPs irrigating solution for elimination of E. faecalis from the root canal system.
CONCLUSIONS: AgNPs activated by ultrasound or PIPS can be used as an adjunct for disinfection of the root canal system in endodontic treatment.
方法:收集并制备59颗拔除的人单根牙齿。将粪肠球菌接种到根管中并孵育4周。然后将牙齿随机分为五个实验组(n=10):AN组,单独使用AgNPs进行灌溉;AN/ICG/DL组,用AgNPs和ICG灌溉,然后用二极管激光器激活;AN/PIPS组,用AgNPs冲洗,用0.3WEr:YAG激光激活;AN/MDA组,用AgNPs灌溉,用锥形古塔胶活化;和AN/PUI组,用AgNPs冲洗并用超声波激活。此外,两个对照组用2.5%次氯酸钠灌溉(n=5)和无干预(n=4)。在干预前后从牙本质芯片中收集样品,计算菌落数减少的百分比。
结果:所有组均显著减少粪肠球菌菌落计数(P<0.05)。在AN/PIPS和AN/PUI组中,菌落计数最大减少了91.03%和91.29%,分别。在单独的AN组中注意到最小的减少。
结论:用PUI和PIPS激活可增强AgNPs冲洗溶液从根管系统中清除粪肠球菌的功效。
结论:经超声或PIPS激活的AgNPs可作为根管治疗中根管系统消毒的辅助手段。