Abstract：
OBJECTIVE: The aim of this study is to measure and compare the amount of apical extrusion of the methylene blue as photosensitizer using Laser-activated irrigation(LAI) techniques (SWEEPS, PIPS) and photodynamic therapy (PDT).
METHODS: 40 single-rooted premolar teeth were selected and their root canals were cleaned and prepared. The photosensitizer, Methylene blue (MB), was used as dye which applied inside the root canals and the access cavities. Then the teeth were randomly allocated to 4 groups. Different techniques (SWEEPS, PIPS, PDT) were used in 3 groups. One group received no activation. Data related to the concentration of the extruded methylene blue was measured using ultraviolet and visible (UV-Vis) absorption spectroscopy. Absorption peak intensity of MB in the wavelength of 668 nm was considered as the concentration index according to Beer Lambert law. Taking into account the normal distribution of this data, one-way ANOVA analysis was used to evaluate the effects of the independent variables on the amount of apical extrusion of the irrigant.
RESULTS: Based on the results of ANOVA analysis, there was no significant difference between the amounts of apical extrusion of MB between different groups (p = 0.628) CONCLUSION: Using SWEEPS and PIPS techniques, despite diffusion of the photosensitizer, didn\'t lead to any difference in the amount of apical extrusion. Therefore, these LAI techniques can be used safely for root canal irrigation.
METHODS: 40 single-rooted premolar teeth were selected and their root canals were cleaned and prepared. The photosensitizer, Methylene blue (MB), was used as dye which applied inside the root canals and the access cavities. Then the teeth were randomly allocated to 4 groups. Different techniques (SWEEPS, PIPS, PDT) were used in 3 groups. One group received no activation. Data related to the concentration of the extruded methylene blue was measured using ultraviolet and visible (UV-Vis) absorption spectroscopy. Absorption peak intensity of MB in the wavelength of 668 nm was considered as the concentration index according to Beer Lambert law. Taking into account the normal distribution of this data, one-way ANOVA analysis was used to evaluate the effects of the independent variables on the amount of apical extrusion of the irrigant.
RESULTS: Based on the results of ANOVA analysis, there was no significant difference between the amounts of apical extrusion of MB between different groups (p = 0.628) CONCLUSION: Using SWEEPS and PIPS techniques, despite diffusion of the photosensitizer, didn\'t lead to any difference in the amount of apical extrusion. Therefore, these LAI techniques can be used safely for root canal irrigation.
摘要：
目的：本研究的目的是使用激光激活灌溉（LAI）技术（SWEEPS，PIPS)和光动力疗法(PDT)。
方法：选择40颗单根前磨牙，清洁并准备其根管。光敏剂，亚甲蓝(MB),用作染料，应用于根管和进入腔内。然后将牙齿随机分为4组。不同的技术(SWEEPS，PIPS,3组采用PDT)。一组未接受激活。使用紫外和可见(UV-Vis)吸收光谱法测量与挤出的亚甲基蓝的浓度相关的数据。根据BeerLambert定律,将MB在668nm波长处的吸收峰强度视为浓度指数。考虑到这些数据的正态分布，单因素方差分析用于评估自变量对冲洗剂顶端挤出量的影响.
结果：基于方差分析的结果，不同组之间的根尖挤出量之间没有显着差异（p=0.628）结论：使用SWEEPS和PIPS技术，尽管光敏剂扩散，没有导致根尖挤压量的任何差异。因此,这些LAI技术可以安全地用于根管冲洗。
方法：选择40颗单根前磨牙，清洁并准备其根管。
结果：基于方差分析的结果，
