Phenazines

吩嗪
  • 文章类型: Journal Article
    Introduction.医院中多重耐药生物(MDRO)的频率以及延迟有效治疗的风险导致几乎所有疑似肺炎患者的呼吸道分泌物培养。培养延迟导致广谱抗生素的过度处方和使用。差距声明。需要改进的快速诊断,以便早期评估疑似医院肺炎。瞄准.要验证新指标,增强型革兰氏染色(EGS),提供高诊断准确性的快速诊断测试,可在疑似肺炎中使用抗生素的临床试验中进行评估。方法论。通过16S核糖体DNA实时聚合酶链反应(16SqPCR)重新测试了先前通过培养和革兰氏染色测试的92份残留下呼吸道样品,并与称为EGS的革兰氏染色相结合。评估EGS的诊断准确性,标准性能测量和与文化的相关性。对于培养物和EGS之间不一致的样品,16S核糖体DNA全操纵子测序(16SrDNAWOS)用于测试分辨率。修正的EGS(A-EGS针对培养进行了重新评估。结果。革兰氏染色,16SqPCR,EGS和A-EGS的诊断准确率分别为77.01%,82.76%,84.04%和94.19%。相同平台与培养物的相关性分别为r=0.67,r=0.71,r=0.81和r=0.89。EGS的阴性预测值(NPV)最高,为93.18%(81.99%-97.62%)。在大多数常规实验室中可以添加16SqPCR结果,结合革兰氏染色,可以改善疑似医院肺炎患者的早期决策。结论。EGS可以改善疑似医院肺炎患者的早期决策,并可以在临床试验中进行评估。A-EGS中的16SrDNAWOS结果也支持在可疑肺炎的早期决策中使用病原体基因组测序。
    Introduction. The frequency of multidrug-resistant organisms (MDROs) in hospitals and the risk of delaying effective treatment result in the culture of respiratory secretions for nearly all patients with suspected pneumonia. Culture delays contribute to over prescribing and use of broader spectrum antibiotics.Gap statement. The need for improved rapid diagnostics for early assessment of suspected hospital pneumonia.Aim. To validate a new metric, enhanced Gram stain (EGS), to provide a rapid diagnostic test of high diagnostic accuracy that could be assessed in clinical trials of the use of antibiotics in suspected pneumonia.Methodology. Ninety-two residual lower respiratory samples previously tested by culture and Gram stain were re-tested by 16S ribosomal DNA real-time polymerase chain reaction (16S qPCR) and reported as a combined metric with Gram stain termed EGS. The EGS was assessed for diagnostic accuracy, standard performance measurements and correlation against culture. For samples with discordance between culture and EGS, 16S ribosomal DNA whole operon sequencing (16S rDNA WOS) was used for test resolution. An amended EGS (A-EGS was reassessed against culture.Results. Gram stain, 16S qPCR, EGS and A-EGS had respective diagnostic accuracies of 77.01 %, 82.76 %, 84.04 % and 94.19 %. The same platforms had respective correlation with culture of r = 0.67, r = 0.71, r = 0.81 and r = 0.89. EGS had the highest negative predictive value (NPV) of 93.18 % (81.99 %-97.62 %). Adding an 16S qPCR result is achievable in most routine laboratories and, combined with Gram stain, could improve early decision-making in patients with suspected hospital pneumonia.Conclusion. EGS could improve early decision-making in patients with suspected hospital pneumonia and could be assessed in clinical trials. The 16S rDNA WOS results in the A-EGS also supported the use of pathogen genomic sequencing in early decision making of suspected pneumonia.
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  • 文章类型: Journal Article
    有才能的内生链霉菌。PH9030来自药用植物Kadsuracoccinea(Lem。)A.C.史密斯。未描述的萘醌萘花碱G(5)和七个先前鉴定的化合物,6-12,从链霉菌sp。PH9030.5的结构通过对其HRESIMS的全面检查来鉴定,1DNMR,2DNMR和ECD数据。研究了所有化合物对α-葡萄糖苷酶的抑制活性及其抗菌性能。首次报道了5、6、7和9的α-葡萄糖苷酶抑制活性,IC50值范围为66.4±6.7至185.9±0.2μM,与阿卡波糖相比(IC50=671.5±0.2μM)。5与α-葡萄糖苷酶的分子对接和分子动力学分析进一步表明其可能与α-葡萄糖苷酶具有良好的结合能力。9和12对耐甲氧西林金黄色葡萄球菌均表现出中等的抗菌活性,最小抑制浓度(MIC)值为16μg/mL。这些结果表明,5,连同萘醌支架,有可能进一步开发作为α-葡萄糖苷酶的可能抑制剂。
    A talented endophytic Streptomyces sp. PH9030 is derived from the medicinal plant Kadsura coccinea (Lem.) A.C. Smith. The undescribed naphthoquinone naphthgeranine G (5) and seven previously identified compounds, 6-12, were obtained from Streptomyces sp. PH9030. The structure of 5 was identified by comprehensive examination of its HRESIMS, 1D NMR, 2D NMR and ECD data. The inhibitory activities of all the compounds toward α-glucosidase and their antibacterial properties were investigated. The α-glucosidase inhibitory activities of 5, 6, 7 and 9 were reported for the first time, with IC50 values ranging from 66.4 ± 6.7 to 185.9 ± 0.2 μM, as compared with acarbose (IC50 = 671.5 ± 0.2 μM). The molecular docking and molecular dynamics analysis of 5 with α-glucosidase further indicated that it may have a good binding ability with α-glucosidase. Both 9 and 12 exhibited moderate antibacterial activity against methicillin-resistant Staphylococcus aureus, with minimum inhibitory concentration (MIC) values of 16 μg/mL. These results indicate that 5, together with the naphthoquinone scaffold, has the potential to be further developed as a possible inhibitor of α-glucosidase.
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  • 文章类型: Journal Article
    背景:花青素是由铜绿假单胞菌产生的蓝色色素。由于其在过去十年中独特的氧化还原特性,作为一种有用的化学品,它获得了越来越多的兴趣。然而,它仍然是一种相当昂贵的试剂。先前已经表明,通过采用各种方法可以提高绿脓苷的产量。其中包括使用统计方法来计划实验或将细菌培养物暴露于应激源,例如以亚致死浓度给药的纳米颗粒,例如氧化锌纳米颗粒。
    结果:实验设计(DoE)方法允许计算最佳工艺温度和纳米颗粒浓度以增强绿脓苷的生产。低浓度的纳米粒子(6.06µg/mL)和32℃的温度增强了绿脓苷的产生,而更高浓度的纳米颗粒(275.75µg/mL)和更高的温度刺激了生物量的产生,并导致了绿脓苷的产生。在放大的培养物中,氧化锌纳米颗粒补充的培养基中色素产量的提高得以维持。进行的分析证实,观察到的绿脓苷生产的刺激随后是更高的膜电位,改变基因表达,产生活性氧,和锌在细胞生物量中的积累。
    结论:可以使用ZnO纳米颗粒来控制氰化素的生产。由于暴露于纳米颗粒而导致的绿脓色素的产生增加是细菌生理变化的数量,并且是细胞应激的结果。我们表明,可以使用统计方法优化细菌的应激反应,并导致更有效地产生所需的代谢物。
    BACKGROUND: Pyocyanin is a blue pigment produced by Pseudomonas aeruginosa. Due to its unique redox properties over the last decade, it has gained more and more interest as a utile chemical. Nevertheless, it remains a rather costly reagent. It was previously shown that the production of pyocyanin can be enhanced by employing various methods. Among them are using statistical methods for planning the experiments or exposing bacterial cultures to stressors such as nanoparticles dosed in sublethal concentrations, e.g. zinc oxide nanoparticles.
    RESULTS: The Design of Experiment (DoE) methodology allowed for calculating the optimal process temperature and nanoparticle concentration to intensify pyocyanin production. Low concentrations of the nanoparticles (6.06 µg/mL) and a temperature of 32℃ enhanced pyocyanin production, whereas higher concentrations of nanoparticles (275.75 µg/mL) and higher temperature stimulated biomass production and caused the abolishment of pyocyanin production. Elevated pigment production in zinc oxide nanoparticles-supplemented media was sustained in the scaled-up culture. Conducted analyses confirmed that observed stimulation of pyocyanin production is followed by higher membrane potential, altered gene expression, generation of reactive oxygen species, and accumulation of zinc in the cell\'s biomass.
    CONCLUSIONS: Pyocyanin production can be steered using ZnO nanoparticles. Elevated production of pyocyanin due to exposure to nanoparticles is followed by the number of changes in physiology of bacteria and is a result of the cellular stress. We showed that the stress response of bacteria can be optimised using statistical methods and result in producing the desired metabolite more effectively.
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  • 文章类型: Journal Article
    细菌色素是具有多功能的特殊天然生物活性化合物。颜料代表来自不同化学类别的分子,包括萜烯,萜类化合物,类胡萝卜素,吡啶,吡咯,吲哚,和吩嗪,由不同的细菌群合成。它们的生理活动范围包括生物活性潜力,通常赋予健身优势,以促进细菌在充满挑战的环境条件下的生存。大部分这样的色素由细菌病原体主要作为次级代谢产物产生。它们的多方面特性增强了生物医学的潜在应用,食物,Pharmaceutical,纺织品,油漆工业,生物修复,以及生物传感器的开发。除了具有对环境有益的属性,对健康的不利影响较小,可操作和可扩展的生产策略使细菌色素成为未开发发现的新型生物技术勘探的可持续选择。该综述全面介绍了细菌病原体色素的生理作用,生产战略,以及在各种生物医学和生物技术领域的潜在应用。旁边,我们讨论了将细菌色素研究与纳米技术等尖端方法相结合的前景,以突出未来的努力。
    Bacterial pigments stand out as exceptional natural bioactive compounds with versatile functionalities. The pigments represent molecules from distinct chemical categories including terpenes, terpenoids, carotenoids, pyridine, pyrrole, indole, and phenazines, which are synthesized by diverse groups of bacteria. Their spectrum of physiological activities encompasses bioactive potentials that often confer fitness advantages to facilitate the survival of bacteria amid challenging environmental conditions. A large proportion of such pigments are produced by bacterial pathogens mostly as secondary metabolites. Their multifaceted properties augment potential applications in biomedical, food, pharmaceutical, textile, paint industries, bioremediation, and in biosensor development. Apart from possessing a less detrimental impact on health with environmentally beneficial attributes, tractable and scalable production strategies render bacterial pigments a sustainable option for novel biotechnological exploration for untapped discoveries. The review offers a comprehensive account of physiological role of pigments from bacterial pathogens, production strategies, and potential applications in various biomedical and biotechnological fields. Alongside, the prospect of combining bacterial pigment research with cutting-edge approaches like nanotechnology has been discussed to highlight future endeavours.
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  • 文章类型: Journal Article
    在这项研究中,结果表明,吩嗪-1羧酸(PCA)诱导铜绿假单胞菌Tet38外排泵表达,引发金黄色葡萄球菌对四环素和吩嗪的耐药。金黄色葡萄球菌RN6390暴露于铜绿假单胞菌PA14及其绿脓苷(PYO)缺陷型突变体的上清液中,表明铜绿假单胞菌非PYO吩嗪可以诱导Tet38外排泵的表达。RN6390以0.25×MIC直接暴露于PCA化合物导致tet38转录本增加五倍。使用RN6390(pRN-tet38p-yfp)通过共聚焦显微镜鉴定Tet38蛋白的表达,该RN6390在PCA的tet38启动子控制下以0.25×MIC表达YFP。Tet38过表达和Δtet38突变体的PCA的MIC显示出三倍的增加和两倍的减少,分别,与野生型相比。在添加四环素(1×或10×MIC)之前,将RN6390预暴露于PCA(0.25×MIC)1小时,细菌生存力提高了1.5倍和2.6倍,分别,但是以10×MIC添加7%的NaCl和四环素可减少2.0-log10CFU/mL的可行PCA暴露RN6390的数量。在PCA和NaCl的存在下,tet38的阻遏物tetR21的转录水平降低和增加了两倍,分别,表明PCA和NaCl对tet38生产的影响是通过TetR21表达发生的。这些数据表明,PCA诱导的Tet38在与铜绿假单胞菌合并感染期间保护金黄色葡萄球菌免受四环素的侵害;然而,诱导的tet38介导的金黄色葡萄球菌对四环素的抗性被NaCl7%逆转,用于增强CF患者痰液动员的雾化治疗。
    In this study, we showed that phenazine-1 carboxylic acid (PCA) of Pseudomonas aeruginosa induced the expression of Tet38 efflux pump triggering Staphylococcus aureus resistance to tetracycline and phenazines. Exposure of S. aureus RN6390 to supernatants of P. aeruginosa PA14 and its pyocyanin (PYO)-deficient mutants showed that P. aeruginosa non-PYO phenazines could induce the expression of Tet38 efflux pump. Direct exposure of RN6390 to PCA compound at 0.25× MIC led to a five-fold increase in tet38 transcripts. Expression of Tet38 protein was identified through confocal microscopy using RN6390(pRN-tet38p-yfp) that expressed YFP under control of the tet38 promoter by PCA at 0.25× MIC. The MICs of PCA of a Tet38-overexpressor and a Δtet38 mutant showed a three-fold increase and a two-fold decrease, respectively, compared with that of wild-type. Pre-exposure of RN6390 to PCA (0.25× MIC) for 1 hour prior to addition of tetracycline (1× or 10× MIC) improved bacteria viability of 1.5-fold and 2.6-fold, respectively, but addition of NaCl 7% together with tetracycline at 10× MIC reduced the number of viable PCA-exposed RN6390 of a 2.0-log10 CFU/mL. The transcript levels of tetR21, a repressor of tet38, decreased and increased two-fold in the presence of PCA and NaCl, respectively, suggesting that the effects of PCA and NaCl on tet38 production occurred through TetR21 expression. These data suggest that PCA-induced Tet38 protects S. aureus against tetracycline during coinfection with P. aeruginosa; however, induced tet38-mediated S. aureus resistance to tetracycline is reversed by NaCl 7%, a nebulized treatment used to enhance sputum mobilization in CF patients.
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  • 文章类型: Journal Article
    抗菌素耐药性已成为威胁人类健康的全球性问题,再加上缺乏新药。金属化合物已成为开发新抗生素的有希望的多种支架。在这里,我们制备了一些金属化合物,主要集中在顺式-[Ru(bpy)(dppz)(SO3)(NO)](PF6)(PR02,bpy=2,2'-联吡啶,dppz=二吡啶[3,2-a:2\',3\'-c]吩嗪),其中菲嗪和一氧化氮配体以及亚硫酸盐赋予了一些关键性质。该化合物对结合的NO+/0表现出-0.252V的氧化还原电势(与Ag|AgCl)和亚硝酰-硝基转化的高pH值为9.16,使亚硝酰配体成为主要物种。这些化合物仍然能够与DNA结构结合。有趣的是,还原型谷胱甘肽(GSH)不能促进显著的NO/HNO释放,许多类似系统的罕见特征。然而,这种还原剂对产生超氧自由基至关重要。使用六种细菌菌株进行了抗菌研究,其中对于革兰氏阴性细菌没有观察到或观察到非常低的活性。然而,PR02和PR(顺式-[Ru(bpy)(dppz)Cl2])在某些革兰氏阳性菌株(金黄色葡萄球菌的MBC高达4.9μmolL-1)中显示出高抗菌活性,其中PR02的活性与PR相似或比PR好至少4倍。此外,PR02显示出抑制细菌生物膜形成的能力,导致细菌对抗生素耐受的主要健康问题。有趣的是,我们还表明,PR02可以与已知的抗生素氨苄青霉素协同作用,提高他们的作用高达4倍,甚至对耐药菌株。总之,这些结果表明,PR02是一种有前途的抗菌亚硝酰钌化合物,结合了其杀伤作用之外的特征,这值得进一步的生物学研究。
    Antimicrobial resistance has become a global threat to human health, which is coupled with the lack of novel drugs. Metallocompounds have emerged as promising diverse scaffolds for the development of new antibiotics. Herein, we prepared some metal compounds mainly focusing on cis-[Ru(bpy)(dppz)(SO3)(NO)](PF6) (PR02, bpy = 2,2\'-bipyridine, dppz = dipyrido[3,2-a:2\',3\'-c]phenazine), in which phenazinic and nitric oxide ligands along with sulfite conferred some key properties. This compound exhibited a redox potential for bound NO+/0 of -0.252 V (vs. Ag|AgCl) and a high pH for nitrosyl-nitro conversion of 9.16, making the nitrosyl ligand the major species. These compounds were still able to bind to DNA structures. Interestingly, reduced glutathione (GSH) was unable to promote significant NO/HNO release, an uncommon feature of many similar systems. However, this reducing agent was essential to generate superoxide radicals. Antimicrobial studies were carried out using six bacterial strains, where none or very low activity was observed for Gram-negative bacteria. However, PR02 and PR (cis-[Ru(bpy)(dppz)Cl2]) showed high antibacterial activity in some Gram-positive strains (MBC for S. aureus up to 4.9 μmol L-1), where the activity of PR02 was similar to or at least 4-fold better than that of PR. Besides, PR02 showed capacity to inhibit bacterial biofilm formation, a major health issue leading to bacterial tolerance to antibiotics. Interestingly, we also showed that PR02 can function in synergism with the known antibiotic ampicillin, improving their action up to 4-fold even against resistant strains. Altogether, these results showed that PR02 is a promising antimicrobial nitrosyl ruthenium compound combining features beyond its killing action, which deserves further biological studies.
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  • 文章类型: Journal Article
    1,4-二甲基吩嗪内过氧化物在37℃下释放单线态氧,半衰期为89小时。热环逆转反应伴随着发射强度的强烈增加,在490nm处有一个峰值,由于吩嗪核心的形成。内过氧化物对培养基和肿瘤球体中的癌细胞有效,具有单线态氧介导的细胞毒性。
    1,4-Dimethylphenazine endoperoxide releases singlet oxygen with a half-life of 89 hours at 37 °C. The thermal cycloreversion reaction is accompanied by a strong increase in the emission intensity with a peak at 490 nm, due to the formation of the phenazine core. The endoperoxide is effective against cancer cells in culture medium and tumor spheroids, with singlet oxygen-mediated cytotoxicity.
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  • 文章类型: Journal Article
    有机磷农药(OPs)的残留正在增加环境污染和公共卫生问题。因此,简单的发展,简便、灵敏的OPs检测方法至关重要。在这里,具有荧光的多功能铁基MOF,催化和吸附,通过简单的一锅法合成。仅使用一种多功能传感材料构建了用于检测OPs的比率荧光传感器。NH2-MIL-101(Fe)能够在H2O2存在下将邻苯二胺(OPD)催化成2,3-二氨基苯并嗪(DAP)。所产生的DAP可以通过荧光共振能量转移(FRET)和内部过滤效应(IFE)显著猝灭NH2-MIL-101(Fe)的固有荧光,同时产生新的可测量的荧光。没有固定或分子印迹,焦磷酸离子(PPi)可以通过与Fe3/Fe2氧化还原对螯合来抑制NH2-MIL-101(Fe)的过氧化物酶样活性。此外,PPi也可以被碱性磷酸酶(ALP)水解,OPs的存在抑制了ALP的活性,导致额外的PPi保存和比率荧光信号变化的增加,通过分子对接探索ALP与不同OPs的相互作用,OPs(例如,草甘膦)与关键氨基酸残基(Asp,Ser,阿拉,示出了Lys和Arg)。所提出的传感器对OPs表现出优异的检测性能,检测极限为18.7nM,这为OPs的检测提供了一种有前途的策略。
    The residues of organophosphorus pesticides (OPs) are increasing environmental pollution and public health concerns. Thus, the development of simple, convenient and sensitive method for detection of OPs is crucial. Herein, a multifunctional Fe-based MOF with fluorescence, catalytic and adsorption, is synthesized by a simple one-pot hydrothermal method. The ratiometric fluorescence sensor for detection of OPs is constructed by using only one multifunctional sensing material. The NH2-MIL-101(Fe) is able catalyze the o-phenylenediamine (OPD) into 2,3-diaminophenazine (DAP) in the presence of H2O2. The generated DAP can significantly quench the intrinsic fluorescence of NH2-MIL-101(Fe) by the fluorescence resonance energy transfer (FRET) and internal filtration effect (IFE), while producing a new measurable fluorescence. Without immobilization or molecular imprinting, pyrophosphate ion (PPi) can inhibit the peroxidase-like activity of the NH2-MIL-101(Fe) by chelating with Fe3+/Fe2+ redox couple. Moreover, PPi can also be hydrolyzed by alkaline phosphatase (ALP), the presence of OPs inhibits the activity of ALP, resulting in the increase of extra PPi preservation and signal changes of ratiometric fluorescence, the interactions of ALP with different OPs are explored by molecular docking, the OPs (e.g., glyphosate) interact with crucial amino acid residues (Asp, Ser, Ala, Lys and Arg) are indicated. The proposed sensor exhibits excellent detection performance for OPs with the detection limit of 18.7 nM, which provides a promising strategy for detection of OPs.
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  • 文章类型: Journal Article
    由禾本科镰刀菌引起的镰刀菌枯萎病是一种严重危害粮食安全和人类健康的小麦病害。先前的研究发现,生控细菌产生的次生代谢产物吩嗪-1-甲酰胺通过与组蛋白乙酰转移酶Gcn5(FgGcn5)结合并抑制其活性来抑制禾谷草原。然而,这种抑制作用的详细机制尚不清楚。我们的结构和生化研究表明,吩嗪-1-甲酰胺(PCN)在其共底物乙酰辅酶A结合位点与FgGcn5的组蛋白乙酰转移酶(HAT)结构域结合,从而竞争性地抑制酶的组蛋白乙酰化功能。由PCN和乙酰辅酶A共享的结合位点中的残基的丙氨酸取代不仅降低了酶的组蛋白乙酰化水平,而且极大地影响了发育。霉菌毒素合成,和毒株的毒力。一起来看,我们的研究阐明了PCN对镰刀菌的竞争性抑制机制,并为设计更有效的吩嗪基杀菌剂提供了结构模板。
    Fusarium head blight caused by Fusarium graminearum is a devastating disease in wheat that seriously endangers food security and human health. Previous studies have found that the secondary metabolite phenazine-1-carboxamide produced by biocontrol bacteria inhibited F. graminearum by binding to and inhibiting the activity of histone acetyltransferase Gcn5 (FgGcn5). However, the detailed mechanism of this inhibition remains unknown. Our structural and biochemical studies revealed that phenazine-1-carboxamide (PCN) binds to the histone acetyltransferase (HAT) domain of FgGcn5 at its cosubstrate acetyl-CoA binding site, thus competitively inhibiting the histone acetylation function of the enzyme. Alanine substitution of the residues in the binding site shared by PCN and acetyl-CoA not only decreased the histone acetylation level of the enzyme but also dramatically impacted the development, mycotoxin synthesis, and virulence of the strain. Taken together, our study elucidated a competitive inhibition mechanism of Fusarium fungus by PCN and provided a structural template for designing more potent phenazine-based fungicides.
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  • 文章类型: Journal Article
    近年来,MXene已成为广泛探索用于各种应用的最吸引人的二维层状(2Dl)材料之一。在这项研究中,开发了Ti3C2MXene/rGo-Cu2O纳米复合材料(TGCNCs),以有效地从水生环境中消除Safranin-O(SO)和酸性品红(AF)作为阳离子染料。多步骤参与吸附剂系统的制备,包括Ti3C2的制备,Humer法合成GO,其次是rGO生产,然后添加CuSO4以获得称为“TGCNCs”的最终纳米复合材料(NCs)。TGCNCs的结构可以以几种方式变化,包括FTIR,SEM,TGA,泽塔,EDX,XRD,而BET,确认TGCNCs的有效制备。开发了一种新型的吸附剂系统来去除SO和AF,两种阳离子染料。通过批量吸附试验优化了各种吸附条件,包括溶液的pH值(4-12),剂量的影响(0.003-0.03克),接触时间的影响(5-30分钟),以及起始染料浓度(25-250mg/L)的影响。因此,TGCNCs表现出对Freundlich等温线模式的极好拟合,产生909.09和769.23毫克的最大AF和SO吸附能力。g-1.对吸附动力学的研究表明,伪二阶(PSO)模型将与实验数据(=0.998和=0.990)很好地拟合。从热力学参数可以明显看出,吸附是自发且有利的吸热过程。在SO和AF吸附到NC上的过程中,假设这些分子通过堆叠相互作用在分子内相互作用,H-键相互作用,静电相互作用,和截留在聚合物Poros结构纳米复合材料内。对于所研究的两种有机染料,最有效的是持续五个循环的再生研究。
    In recent years, MXene has become one of the most intriguing two-dimensional layered (2Dl) materials extensively explored for various applications. In this study, a Ti3C2 MXene/rGo-Cu2O Nanocomposite (TGCNCs) was developed to eliminate Safranin-O effectively (SO) and Acid Fuchsin (AF) as cationic dyes from the aquatic environment. Multistep was involved in the preparation of the adsorbent system, including the Preparation of Ti3C2, after that, GO synthesis by the Humer method, followed by rGO production, then added CuSO4 to obtain a final Nanocomposite (NCs) called \"TGCNCs\". The structure of TGCNCs can be varied in several ways, including FTIR, SEM, TGA, Zeta, EDX, XRD, and BET, to affirm the efficacious preparation of TGCNCs. A novel adsorbent system was developed to remove SO and AF, both cationic dyes. Various adsorption conditions have been optimized through batch adsorption tests, including the pH of the solution (4-12), the effect of dosage (0.003-0.03 g), the impact of the contact time (5-30 min), and the effect of beginning dye concentration (25-250 mg/L). Accordingly, the TGCNCs exhibited excellent fitting for Freundlich isotherm mode, resulting in maximum AF and SO adsorption capacities of 909.09 and 769.23 mg g-1. This research on adsorption kinetics suggests that a pseudo-second-order (PSO) model would fit well with the experimental data (RSO2 = 0.998 and RAF2 = 0.990). It is evident from the thermodynamic parameters that adsorption is an endothermic process that is spontaneous and favorable. During the adsorption of SO and AF onto NCs, it is hypothesized that these molecules interact intramolecularly through stacking interactions, H-bond interactions, electrostatic interactions, and entrapment within the polymeric Poros structure nanocomposite. Regeneration studies lasting up to five cycles were the most effective for both organic dyes under study.
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