急性炎症过程由许多假设解释,包括氧化应激,酶刺激,和促炎细胞因子的产生。研究了丝兰甲醇提取物(YGME)对角叉菜胶诱导的急性炎症的抗炎活性及其可能的潜在机制。植物化学概况,细胞毒性,和抗菌活性也进行了探索。LC-MS/MS用于研究YGME的化学成分,并初步鉴定出29个化合物。此外,木犀草素-7-O-β-d-葡萄糖苷的分离,芹菜素-7-O-β-d-葡萄糖苷,山奈酚-3-O-α-1-鼠李糖苷是首次从研究的植物中获得。通过皮下注射100μL的1%角叉菜胶钠诱导炎症。大鼠口服YGME100,200mg/kg,塞来昔布(50mg/kg),和生理盐水,分别,注射角叉菜胶前一小时.在几个时间间隔测量爪子水肿的平均体积和重量。NO的水平,GSH,TNF-α,PGE-2,血清IL-1β,测量IL-6。此外,对爪组织进行COX-2免疫染色和组织病理学检查。YGME通过减少爪子水肿表现出有效的抗炎作用,PGE-2,TNF-α,不生产,血清IL-6,IL-1β,和COX-2免疫染色。此外,它补充了减少的爪子GSH含量并改善了组织病理学发现。YGME的最佳细胞毒性作用是针对人黑色素瘤细胞系(A365)和骨肉瘤细胞系(MG-63)。此外,针对细菌和真菌分离株评估了提取物的抗菌潜力。它对革兰氏阴性显示出有效的活性,革兰氏阳性,和真菌白色念珠菌分离株。促进YGME的多重作用可能是有益的治疗不同疾病的基础上,其抗炎,抗菌,和细胞毒性作用。
The acute inflammation process is explained by numerous hypotheses, including oxidative stress, enzyme stimulation, and the generation of pro-inflammatory cytokines. The anti-inflammatory activity of Yucca gigantea methanol extract (YGME) against carrageenan-induced acute inflammation and possible underlying mechanisms was investigated. The phytochemical profile, cytotoxic, and antimicrobial activities were also explored. LC-MS/MS was utilized to investigate the chemical composition of YGME, and 29 compounds were tentatively identified. In addition, the isolation of luteolin-7-O-β-d-glucoside, apigenin-7-O-β-d-glucoside, and kaempferol-3-O-α-l-rhamnoside was performed for the first time from the studied plant. Inflammation was induced by subcutaneous injection of 100 μL of 1% carrageenan sodium. Rats were treated orally with YGME 100, 200 mg/kg, celecoxib (50 mg/kg), and saline, respectively, one hour before carrageenan injection. The average volume of paws edema and weight were measured at several time intervals. Levels of NO, GSH, TNF-α, PGE-2, serum IL-1β, IL-6 were measured. In additionally, COX-2 immunostaining and histopathological examination of paw tissue were performed. YGME displayed a potent anti-inflammatory influence by reducing paws edema, PGE-2, TNF-α, NO production, serum IL-6, IL-1β, and COX-2 immunostaining. Furthermore, it replenished the diminished paw GSH contents and improved the histopathological findings. The best cytotoxic effect of YGME was against human melanoma cell line (A365) and osteosarcoma cell line (MG-63). Moreover, the antimicrobial potential of the extract was evaluated against bacterial and fungal isolates. It showed potent activity against Gram-negative, Gram-positive, and fungal Candida albicans isolates. The promoting multiple effects of YGME could be beneficial in the treatment of different ailments based on its anti-inflammatory, antimicrobial, and cytotoxic effects.