本研究旨在评价九种药材天然提取物(SMA)对其生长性能的影响,豁免权,实验感染柔嫩艾美耳球虫的肉鸡的肠道完整性。将252只1日龄肉鸡分为7组,每组3只,每笼12只肉鸡。各组均为未感染-未治疗的空白对照组(BC),未经感染的阴性对照组(NC),SMA治疗组,中药阳性对照组(CM),和化学药物阳性对照组(CD)。SMA组感染并饲喂补充0.6(SMA-L)的基础饮食,0.8(SMA-M),和1.0(SMA-H)g/kgSMA。CM和CD组感染并饲喂基础日粮,补充15g/kg吉秋冲散和0.2g/kg双克拉齐脲,分别。结果表明,饲喂SMA可以显著减少感染鸡的卵囊数量,特别是1.0g/kgSMA,表现出中等的抗球虫功效。当感染了E.tenella时,补充1.0g/kgSMA增加了肾脏指数;恢复了肝脏,脾,和法氏囊指数对BC水平的影响;增加了免疫球蛋白A(IgA)的水平,IgM,和IgY;并降低肿瘤坏死因子(TNF-α)的含量,干扰素-γ(IFN-γ),白细胞介素-6(IL-6),和感染鸡的IL-10。此外,1.0g/kgSMA治疗减轻了盲肠组织的病理变化,增加了小带囊1(ZO-1)的含量,occludin,E.tenella感染的鸡的盲肠组织中的claudin-1和粘蛋白2(粘蛋白2)。我们发现1.0g/kgSMA减少了卵囊的数量,提高免疫力,减轻肠屏障的损伤,可以提高感染鸡的生长性能。因此,SMA被证明是针对E.tenella的有效天然提取物,并有可能用作有效的抗球虫药物或添加剂。
This study aimed to evaluate the effects of natural extracts from nine medicinal herbs (SMA) on the growth performance, immunity, and intestinal integrity of broilers experimentally infected with Eimeria tenella. A total of 252 one-day-old broiler chicks were divided into 7 groups with 3 replicates per group and 12 broilers per cage. The groups were uninfected-untreated blank control group (BC), infected-untreated negative control group (NC), SMA treatment groups, Chinese medicine positive control group (CM), and chemical drug positive control group (CD). The SMA groups were infected and fed a basal diet supplemented with 0.6 (SMA-L), 0.8 (SMA-M), and 1.0 (SMA-H) g/kg SMA. The CM and CD groups were infected and fed a basal diet supplemented with 15 g/kg Jiqiuchong San and 0.2 g/kg Diclazuril, respectively. Results showed that feeding SMA could significantly reduce the number of oocysts in infected chickens, especially 1.0 g/kg SMA, which exhibited moderate anticoccidial efficacy. When infected with E. tenella, the supplementation of 1.0 g/kg SMA increased the renal index; restored the hepatic, splenic, and bursal indexes to BC levels; increased the levels of immunoglobulin A (IgA), IgM, and IgY; and reduced the contents of tumor necrosis factor (TNF-α), interferon-γ (IFN-γ), interleukin-6 (IL-6), and IL-10 of the infected chickens. Moreover, treatment with 1.0 g/kg SMA alleviated the pathological changes in cecal tissue and increased the contents of zonula occludens-1 (ZO-1), occludin, claudin-1, and mucoprotein 2 (mucin-2) in cecal tissues of E. tenella-infected chickens. We found that 1.0 g/kg SMA reduced the number of oocysts, improved immunity, and alleviated intestinal barrier damage, which could improve the growth performance of infected chickens. Thus, SMA proved to be an effective natural extract against E. tenella and has the potential to be used as an efficient anticoccidial drug or additive.