Abstract：
Glycyrrhiza glabra extract is widely known for its antioxidant and anti-inflammatory properties and can improve the wound healing process. The aim of this work was to shorten the time of the healing process by using an eco-sustainable wound dressing based on Spanish broom flexible cellulosic fabric by impregnation with G. glabra extract-loaded ethosomes. Chemical analysis of G. glabra extract was performed by LC-DAD-MS/MS and its encapsulation into ethosomes was obtained using the ethanol injection method. Lipid vesicles were characterized in terms of size, polydispersity index, entrapment efficiency, zeta potential, and stability. In vitro release studies, biocompatibility, and scratch test on 3T3 fibroblasts were performed. Moreover, the structure of Spanish broom dressing and its ability to absorb wound exudate was characterized by Synchrotron X-ray phase contrast microtomography (SR-PCmicroCT). Ethosomes showed a good entrapment efficiency, nanometric size, good stability over time and a slow release of polyphenols compared to the free extract, and were not cytotoxic. Lastly, the results revealed that Spanish broom wound dressing loaded with G. glabra ethosomes is able to accelerate wound closure by reducing wound healing time. To sum up, Spanish broom wound dressing could be a potential new green tool for biomedical applications.
摘要：
甘草提取物因其抗氧化和抗炎特性而广为人知，可以改善伤口愈合过程。这项工作的目的是通过使用基于西班牙扫帚柔性纤维素织物的生态可持续伤口敷料，通过浸渍G.glabra提取物负载的乙醇体，缩短愈合过程的时间。通过LC-DAD-MS/MS进行G.glabra提取物的化学分析,并且使用乙醇注射方法获得其在醇质体中的包封。脂质囊泡的特征在于大小，多分散指数，截留效率，zeta电位,和稳定性。体外释放研究，生物相容性，对3个T3成纤维细胞进行划痕试验。此外，西班牙扫帚敷料的结构及其吸收伤口渗出物的能力通过同步加速器X射线相衬显微断层扫描（SR-PCmicroCT）进行了表征。乙醇体表现出良好的包封效率，纳米尺寸，与游离提取物相比，随着时间的推移具有良好的稳定性和多酚的缓慢释放，并且没有细胞毒性。最后,结果表明，载有G.glabra醇质体的西班牙扫帚伤口敷料能够通过减少伤口愈合时间来加速伤口闭合。总而言之,西班牙扫帚伤口敷料可能是生物医学应用的潜在新绿色工具。
