囊肿线虫被认为是各种主要粮食作物产量的主要威胁。当前的控制策略主要取决于作物轮作和抗性品种的使用。各种作物表现出单个显性抗性(R)基因,其能够激活对某些胞囊线虫物种和/或种群的有效宿主特异性抗性。一个例子是马铃薯R基因Gpa2,它赋予对马铃薯囊肿线虫(PCN)的抗性,PallidaGlobodera种群D383。Gpa2的激活导致延迟的抗性响应,其特征是在发育中的线虫摄食结构周围形成一层坏死细胞。然而,关于Gpa2诱导的防御途径的知识仍然缺乏。这里,我们揭示了Gpa2激活诱导的转录变化和基因表达网络在马铃薯根中感染了G.pallida。为此,体外培养的抗Gpa2马铃薯根受无毒种群D383和有毒种群Rookmaker感染。在3和6dpi收获感染的根段,并送去进行RNA测序。比较转录组学显示线虫感染后总共有1,743个差异表达基因(DEG),其中559个DEGs被特异性调节以响应D383感染。与Gpa2介导的防御相关的D383特异性DEGs主要与钙结合活性有关,水杨酸(SA)生物合成,和系统获得性抵抗(SAR)。这些数据表明,马铃薯根中的囊肿线虫抗性取决于植物免疫中保守的下游信号通路,它们也已知有助于R基因介导的对具有不同生活方式的其他病原体的抗性。
Cyst nematodes are considered a dominant threat to yield for a wide range of major food crops. Current control strategies are mainly dependent on crop rotation and the use of resistant cultivars. Various crops exhibit single dominant resistance (R) genes that are able to activate effective host-specific resistance to certain cyst nematode species and/or populations. An example is the potato R gene Gpa2, which confers resistance against the potato cyst nematode (PCN), Globodera pallida population D383. Activation of Gpa2 results in a delayed resistance response, which is characterized by a layer of necrotic cells formed around the developing nematode feeding structure. However, knowledge about the Gpa2-induced defense pathways is still lacking. Here, we uncover the transcriptional changes and gene expression network induced upon Gpa2 activation in potato roots infected with G. pallida. To this end, in vitro-grown Gpa2-resistant potato roots were infected with the avirulent population D383 and virulent population Rookmaker. Infected root segments were harvested at 3 and 6 dpi and sent for RNA sequencing. Comparative transcriptomics revealed a total of 1,743 differentially expressed genes (DEGs) upon nematode infection, of which 559 DEGs were specifically regulated in response to D383 infection. D383-specific DEGs associated with Gpa2-mediated defense mainly relates to calcium-binding activity, salicylic acid (SA) biosynthesis, and systemic acquired resistance (SAR). These data reveal that cyst nematode resistance in potato roots depends on conserved downstream signaling pathways involved in plant immunity, which are also known to contribute to R genes-mediated resistance against other pathogens with different lifestyles.