该研究的目的是研究8种商业饮用水添加剂对主要人畜共患病家禽病原体(弯曲杆菌属。,大肠杆菌,鼠伤寒沙门氏菌,金黄色葡萄球菌和李斯特菌。).我们测试了两种基于精油的植物遗传学(PhytoCSCLiquideB,AEN350B液体),两种酸基益生菌(Salgard®液体,Intesti-Flora),和四种精油和有机酸的混合物(ProPhorceTMSAExclusive,草药酸,Rigosol-N和Eubisan3000)。通过使用微量稀释法估算最小抑制浓度(MIC)来确定抗菌活性。产品对弯曲杆菌属的MIC。范围从0.071%到0.568%v/v,其中草药酸,富含乳酸和磷酸的混合物,还含有百里香和牛至油,表现出对所有测试菌株的最高效力(MIC:0.071%v/v)。测试产品对大肠杆菌的MIC范围为0.071%至1.894%v/v。具体来说,Rigosol-N的MIC,高浓度乳酸和乙酸的混合物,两种测试菌株的v/v为0.142%,而肠道植物区系的中等收入国家,富含乳酸和丙酸的混合物,范围从0.284%到0.568%v/v。产品对鼠伤寒沙门氏菌的MIC在0.095%和1.894%v/v之间。具体来说,Eubisan3000的MIC,一种富含牛至油的混合物,是0.284%v/v对金黄色葡萄球菌的MIC在0.142%和9.090%v/v之间。PhytoCSC液化B的MIC,富含反式肉桂醛,在3.030%和9.090%v/v之间,显示所有测试产品的最高MIC值。最后,测试的商业产品对李斯特菌的MIC值。为0.095%至3.030%v/v。ProPhorceTMSA独家的MIC,甲酸及其盐的高度浓缩混合物,对李斯特菌为0.095-0.142%v/v。,而AEN350B液体的MIC在0.284%至1.894%之间,表现出高李斯特菌。应变变异性。总之,所有选定的商业产品都或多或少地表现出对病原菌的抗菌活性,因此,可以作为抗生素的替代品,用于控制人畜共患家禽病原体和限制抗微生物细菌。
The aim of the study was to investigate in vitro the antibacterial activity of 8 commercial drinking water additives against major zoonotic poultry pathogens (Campylobacter spp., Escherichia coli, Salmonella Typhimurium, Staphylococcus aureus and Listeria spp.). We tested two essential oil-based phytogenics (Phyto CSC Liquide B, AEN 350 B Liquid), two acid-based eubiotics (Salgard® liquid, Intesti-Flora), and four blends of essential oils and organic acids (ProPhorceTM SA Exclusive, Herbal acid, Rigosol-N and Eubisan 3000). The antibacterial activity was determined by estimating the minimum inhibitory concentration (MIC) using a microdilution method. The MICs of the products against Campylobacter spp. ranged from 0.071% to 0.568% v/v, in which Herbal acid, a blend rich in lactic and phosphoric acids, also containing thyme and oregano oils, exhibited the highest efficacy (MIC: 0.071% v/v) against all the tested strains. The MICs of the tested products against Escherichia coli ranged between 0.071% and 1.894% v/v. Specifically, the MIC of Rigosol-N, a blend of high concentrations of lactic and acetic acid, was 0.142% v/v for both tested strains, whereas the MICs of Intesti-Flora, a mixture rich in lactic and propionic acid, ranged from 0.284% to 0.568% v/v. The MICs of the products against Salmonella Typhimurium were between 0.095% and 1.894% v/v. Specifically, the MIC of Eubisan 3000, a blend rich in oregano oil, was 0.284% v/v. The MICs against Staphylococcus aureus were between 0.142% and 9.090% v/v. The MICs of Phyto CSC Liquide B, which is rich in trans-cinnamaldehyde, were between 3.030% and 9.090% v/v, showing the highest MIC values of all tested products. Finally, the MIC values of the tested commercial products against Listeria spp. were 0.095% to 3.030% v/v. The MICs of ProPhorceTM SA Exclusive, a highly concentrated blend of formic acid and its salts, were 0.095-0.142% v/v against Listeria spp., while the MICs of AEN 350 B Liquid were between 0.284% and 1.894% exhibiting high Listeria spp. strain variability. In conclusion, all the selected commercial products exhibited more or less antibacterial activity against pathogenic bacteria and, thus, can be promising alternatives to antibiotics for the control of zoonotic poultry pathogens and the restriction of antimicrobial-resistant bacteria.