PDF(Pubmed)
Abstract：
Antimicrobial resistance is becoming a problem of concern in the veterinary field, necessitating the use of effective topical treatments to aid the healing of wounds. Honey has been used for thousands of years for its medicinal properties, but in recent years medical-grade Manuka honey has been used to treat infected wounds. The goal of this study was to determine the relative susceptibility of four common equine wound pathogens to ten different types of antimicrobial agents based on the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The pathogens studied include ATCC lab-acclimated Pseudomonas aeruginosa, Escherichia coli, and methicillin-resistant Staphylococcus aureus and one from an equine sample submitted to the Colorado State Veterinary Diagnostic Laboratory (Streptococcus equi ssp. zooepidemicus (Streptococcus zooepidemicus)). An additional goal of the study was to describe the comparison of bactericidal activity of medical-grade Manuka honey, local honey, and commercial, food-grade honey to other commonly used wound dressings (20% hypertonic saline, silver sulfadiazine cream, PHMB gauze, and PHMB foam). The objective is to provide veterinary practitioners with comparative data on the use of a variety of antimicrobial dressings for inhibiting the growth of common wound bacteria. MIC and MBC for Manuka, store, and local honeys were comparable to those of sterile gauze, sugar, and hypertonic saline. Across bacterial species, local honey proved to have more bactericidal activity when compared to Manuka honey and commercial, food-grade honey. The MIC and MBC for PHMB gauze and foam was consistently at a higher dilution compared to the other antimicrobials. The majority of antimicrobials exhibited stronger inhibitory and bactericidal activity against a Streptococcus zooepidemicus isolate obtained from a wound compared to other bacteria that were ATCC lab-acclimated. Additional research for in vivo applications needs to be done to see whether differences exist in effective wound management.
摘要：
抗菌素耐药性正在成为兽医领域关注的问题，需要使用有效的局部治疗来帮助伤口愈合。蜂蜜因其药用特性已经使用了数千年，但近年来，医用级麦卢卡蜂蜜已被用于治疗感染的伤口。这项研究的目的是根据最低抑菌浓度（MIC）和最低杀菌浓度（MBC）确定四种常见马伤口病原体对十种不同类型抗菌剂的相对敏感性。研究的病原体包括ATCC实验室驯化的铜绿假单胞菌，大肠杆菌,和耐甲氧西林金黄色葡萄球菌，以及来自提交给科罗拉多州兽医诊断实验室的马样本中的一种（马氏链球菌。动物流行病（动物流行病链球菌））。该研究的另一个目标是描述医用级麦卢卡蜂蜜的杀菌活性的比较，当地蜂蜜,商业,食品级蜂蜜与其他常用的伤口敷料(20%高渗盐水，磺胺嘧啶银乳膏，PHMB纱布,和PHMB泡沫）。目的是为兽医从业人员提供关于使用多种抗微生物敷料来抑制常见伤口细菌生长的比较数据。麦卢卡的MIC和MBC，store,当地蜂蜜与无菌纱布相当，糖,和高渗盐水.在细菌物种中，与麦卢卡蜂蜜和商业蜂蜜相比，当地蜂蜜被证明具有更多的杀菌活性，食品级蜂蜜.与其他抗微生物剂相比,PHMB纱布和泡沫的MIC和MBC始终处于较高的稀释度。与ATCC实验室适应的其他细菌相比，大多数抗微生物剂对从伤口获得的兽疫链球菌分离物表现出更强的抑制和杀菌活性。需要对体内应用进行其他研究，以了解有效的伤口处理是否存在差异。
