DennettiatripetalaG.Baker(番荔枝科),是一种有营养的植物,社会经济,和药用价值。其不断增长的药用特性使这种植物成为药物发现的前景。然而,据报道,习惯性消费者具有很强的成瘾潜力,因此必须建立其安全性。在这份报告中,我们使用体内单剂量和重复剂量毒性分析评估了未产雌性Wistar大鼠中D.tripetala种子精油(EODS)的安全性,以及其已知种子油来源的植物成分的硅毒性分析。我们的结果表明,相对体重的剂量依赖性变化一致,器官-身体和器官-大脑的重量比,血液学和生化指标,以及肝脏和肾脏的组织结构,在单次和重复口服给药之后。肝脏和肾脏组织结构的显着改变与观察到的AST/ALT比率显着增加一致,提示EODS对肾脏和肝脏的有害影响。然而,海马和下丘脑的组织结构没有改变,这表明大脑可能没有受到不利影响。此外,计算机模拟分析表明,EODS的肝毒性作用可能与苄腈有关,Humulene,芳樟醇,(Z)-β-辛烯。此外,β-苯基硝基乙烷的失效,EODS中最丰富的植物成分,通过计算机毒性筛选的第一阶段和第二阶段,以及石竹烯氧化物的存在,一种已知的有毒化合物,结合预测的DNA和蛋白质的结合,在250mg/kg的重复剂量下,低LD50和高死亡率,进一步证实了EODS的潜在毒性。我们得出的结论是,根据我们的体内和电脑观察,迫切需要公共教育来规范D.tripetala种子的过度消费。
Dennettia tripetala G. Baker (Annonaceae), is a plant with nutritional, social economy, and medicinal values. Its rising medicinal profile makes this plant a prospect in drug discovery. However, the reported strong addictive potential among habitual consumers makes the need to establish its safety imperative. In this report, we evaluated the safety profile of the essential oil of the seed of D. tripetala (EODS) in nulliparous female Wistar rats using in vivo single and repeated dose toxicity profiling, as well as in silico toxicity profiling of its known seed oil derived phytoconstituents. Our results showed consistent significant dose-dependent alterations in relative body weights, organ-body and organ-brain weight ratios, haematological and biochemical indices, as well as liver and kidney histoarchitectures, following single and repeated oral administrations. Significant alterations in liver and kidney histoarchitectures were consistent with the observed significant increase in AST/ALT ratio, suggesting deleterious effects of EODS on the kidney and liver. However, the lack of alterations in the histoarchitectures of the hippocampus and hypothalamus suggests that the brain may not have been adversely affected. Also, the in silico analysis suggests that hepatotoxic effects of EODS may be linked to Benzylnitrile, Humulene, Linalool, (Z)-ß-Ocimene. In addition, the failure of ß-Phenylnitroethane, the most abundant phytoconstituent of EODS, to pass phases I and II in silico toxicity screening, and the presence of Caryophyllene oxide, a known toxic compound, coupled with the predicted binding of both to DNA and protein, low LD50 and high percent mortality at 250 mg/kg of repeated doses, further confirmed the potentially toxic nature of EODS. We concluded that based on our in vivo and in silico observations, there is an urgent need for public education to regulate the excessive consumption of the seeds of D. tripetala.