背景:作物相关微生物在土壤养分循环中起着至关重要的作用,和作物生长,和健康。土壤微生物群落多样性和组成的精细尺度模式通常受植物物种或基因型调节。尽管广泛报道了不同作物或其品种对微生物群落的影响,不确定花生(RP,光滑蜘蛛。),一种常年温暖季节的豆科牧草,在美国南部很好地适应,影响不同品种的土壤微生物群落。
结果:本研究探讨了七个不同RP品种对分类组成的影响,多样性,通过在玛丽安娜的田间试验,以及土壤真菌群落的功能群,佛罗里达,美国南部,使用下一代测序技术。我们的结果表明,不同RP品种的真菌群落的分类多样性和组成存在显着差异。阿尔法多样性(香农,辛普森,和Pielou的均匀度)在Ecoturf中明显较高,但在UF_Peace和Florigraze中明显较低(p<0.001)。与其他品种相比,纬度地区的系统发育多样性(Faith'sPD)最低(p<0.0001)。占优势的门是子囊(13.34%),被孢霉(3.82%),担子菌(2.99%),在弗洛里格拉兹明显更大,UF_和平,还有Ecoturf,分别。在UF_Tito中,新孢子的相对丰度明显较高(21.45%),并且在不同品种之间表现出很大的差异。Arbrook的优势属的相对丰度明显高于其他品种。共现网络也存在显著差异,各品种之间表现出不同的梯形分类群和比负相关更多的正相关。FUNGuild分析表明,功能公会的相对丰度包括致病性,腐化,内生植物,菌根和寄生真菌在品种之间存在显着差异。Ecoturf的菌根真菌组相对丰度最大(5.10±0.44),与其他品种相比,UF_Peace的内生真菌(4.52±0.56)和寄生真菌(1.67±0.30)的相对丰度最高。
结论:我们的发现提供了证据,证明了作物品种在豆科植物饲草系统中形成细尺度真菌群落模式的作用。
BACKGROUND: Crop-associated microorganisms play a crucial role in soil nutrient cycling, and crop growth, and health. Fine-scale patterns in soil microbial community diversity and composition are commonly regulated by plant species or genotype. Despite extensive reports in different crop or its cultivar effects on the microbial community, it is uncertain how rhizoma peanut (RP, Arachis glabrata Benth.), a perennial warm-season legume forage that is well-adapted in the southern USA, affects soil microbial community across different cultivars.
RESULTS: This study explored the influence of seven different RP cultivars on the taxonomic composition, diversity, and functional groups of soil fungal communities through a field trial in Marianna, Florida, Southern USA, using next-generation sequencing technique. Our results showed that the taxonomic diversity and composition of the fungal community differed significantly across RP cultivars. Alpha diversity (Shannon, Simpson, and Pielou\'s evenness) was significantly higher in Ecoturf but lower in UF_Peace and Florigraze compared to other cultivars (p < 0.001). Phylogenetic diversity (Faith\'s PD) was lowest in Latitude compared to other cultivars (p < 0.0001). The dominant phyla were Ascomycota (13.34%), Mortierellomycota (3.82%), and Basidiomycota (2.99%), which were significantly greater in Florigraze, UF_Peace, and Ecoturf, respectively. The relative abundance of Neocosmospora was markedly high (21.45%) in UF_Tito and showed large variations across cultivars. The relative abundance of the dominant genera was significantly greater in Arbrook than in other cultivars. There were also significant differences in the co-occurrence network, showing different keystone taxa and more positive correlations than the negative correlations across cultivars. FUNGuild analysis showed that the relative abundance of functional guilds including pathogenic, saprotrophic, endophytic, mycorrhizal and parasitic fungi significantly differed among cultivars. Ecoturf had the greatest relative abundance of mycorrhizal fungal group (5.10 ± 0.44), whereas UF_Peace had the greatest relative abundance of endophytic (4.52 ± 0.56) and parasitic fungi (1.67 ± 0.30) compared to other cultivars.
CONCLUSIONS: Our findings provide evidence of crop cultivar\'s effect in shaping fine-scale fungal community patterns in legume-based forage systems.