PDF(Pubmed)
Abstract：
Cowpeas (Vigna unguiculata L. Walp) have been credible constituents of nutritious food and forage in human and animal diets since the Neolithic era. The modern technique of Diversity Array Technology (DArTseq) is both cost-effective and rapid in producing thousands of high-throughputs, genotyped, single nucleotide polymorphisms (SNPs) in wide-genomic analyses of genetic diversity. The aim of this study was to assess the heterogeneity in cowpea genotypes using DArTseq-derived SNPs. A total of 92 cowpea genotypes were selected, and their fourteen-day-old leaves were freeze-dried for five days. DNA was extracted using the CTAB protocol, genotyped using DArTseq, and analysed using DArTsoft14. A total of 33,920 DArTseq-derived SNPs were recalled for filtering analysis, with a final total of 16,960 SNPs. The analyses were computed using vcfR, poppr, and ape in R Studio v1.2.5001-3 software. The heatmap revealed that the TVU 9596 (SB26), Orelu (SB72), 90K-284-2 (SB55), RV 403 (SB17), and RV 498 (SB16) genotypes were heterogenous. The mean values for polymorphic information content, observed heterozygosity, expected heterozygosity, major allele frequency, and the inbreeding coefficient were 0.345, 0.386, 0.345, 0.729, and 0.113, respectively. Moreover, they validated the diversity of the evaluated cowpea genotypes, which could be used for potential breeding programmes and management of cowpea germplasm.
摘要：
自新石器时代以来，豌豆（VignaunguiculataL.Walp）一直是人类和动物饮食中营养食品和饲料的可靠成分。多样性阵列技术（DArTseq）的现代技术既具有成本效益，又可以快速产生数千个高吞吐量，基因分型，单核苷酸多态性（SNP）在遗传多样性的广泛基因组分析。这项研究的目的是使用DArTseq衍生的SNP评估cow豆基因型的异质性。总共选择了92个of豆基因型，他们十四天大的叶子被冻干五天。使用CTAB方案提取DNA，使用DArTseq进行基因分型，并使用DArTSoft14进行分析。总共召回了33,920个DArTseq衍生的SNP用于过滤分析，最终共有16,960个SNP。分析是使用vcfR计算的，poppr,和猿在RStudiov1.2.5001-3软件。热图显示TVU9596(SB26)，Orelu(SB72),90K-284-2(SB55),RV403(SB17),RV498（SB16）基因型具有异质性。多态信息内容的平均值，观察到的杂合性，预期杂合性，主要等位基因频率，近交系数分别为0.345、0.386、0.345、0.729和0.113。此外，他们验证了所评估的cow豆基因型的多样性，可用于潜在的育种计划和of豆种质的管理。
