未经授权:氨基酸转运蛋白是已知介导氨基酸转移的跨膜蛋白。作为氨基酸转运蛋白之一,由Slc7a5编码的LAT1介导必需氨基酸的细胞摄取。最近,大多数研究都集中在研究LAT1与骨骼发育之间的关系。然而,关于软骨中LAT1的临床特征知之甚少,这可能导致脊柱侧弯等骨骼畸形的发展。因此,这项研究的目的是研究儿童脊柱侧凸患者L型氨基酸转运蛋白1(LAT1)及其溶质载体转运蛋白7a5(Slc7a5)的表达,并比较LAT1和Slc7a5表达与临床特征的关系。
UNASSIGNED:我们前瞻性招募了56例脊柱侧凸矫正脊柱融合术患者。患者包括40名女孩和16名男孩,手术时平均年龄为13.1岁。有34例特发性脊柱侧凸(IS)患者,先天性脊柱侧凸(CS)患者22例。在手术过程中,收获了根尖椎骨棘突的骨phy部分;然后,评估LAT1和Slc7a5在软骨中的表达。
未经批准:根据我们的发现,在60.7%(56个中的34个)的患者的软骨中观察到LAT1表达。LAT1在IS患者中的表达率为76%,统计学上高于CS患者的36%。当与LAT1表达比较时,在年龄方面没有统计学差异,性别,体重指数(BMI),Cobb角,和Risser等级。同时,确定IS患者中Slc7a5的平均表达显著高于CS患者.Slc7a5表达与年龄无显著相关性,BMI,和Cobb角。
UNASSIGNED:LAT1和Slc7a5在IS和CS患者中的表达有统计学差异。发现这些表达与年龄无关,身材,以及畸形的严重程度。
UNASSIGNED: Amino acid transporters are transmembrane proteins that are known to mediate the transfer of amino acids. As one of the amino acid transporters, LAT1, which is encoded by Slc7a5, mediates the cellular uptake of the essential amino acids. Recently, most studies have focused on examining the relationship between LAT1 and skeletal formation in terms of development. However, little is known regarding the clinical features of LAT1 in the cartilage, which might result in the development of skeletal deformities such as scoliosis. Thus, the aim of this study was to investigate the expression of L-type amino acid transporter 1 (LAT1) and its solute carrier transporter 7a5 (Slc7a5) in patients with pediatric scoliosis and to compare with the relationship between LAT1 and Slc7a5 expression and their clinical features.
UNASSIGNED: We have prospectively recruited 56 patients who underwent corrective spinal fusion for scoliosis. The patients comprised 40 girls and 16 boys, with a mean age of 13.1 years at the time of surgery. There were 34 idiopathic scoliosis (IS) patients, whereas 22 were congenital scoliosis (CS) patients. During the surgery, an epiphyseal part of the spinous process at apical vertebra was harvested; then, LAT1 and Slc7a5 expressions in the cartilage were evaluated.
UNASSIGNED: As per our findings, LAT1 expression was observed in the cartilage in 60.7% (34 out of 56) of the patients. LAT1 expression in IS patients was 76%, which were statistically higher compared to 36% in CS patients. When compared with LAT1 expression, no statistical difference was noted in terms of age, gender, body mass index (BMI), Cobb angle, and Risser grade. Meanwhile, the mean Slc7a5 expression in IS patients was determined to be significantly higher than that in CS patients. No significant correlation was observed between Slc7a5 expression and age, BMI, and Cobb angle.
UNASSIGNED: LAT1 and Slc7a5 expression in IS and CS patients showed significant differences. These expressions were found to be not correlated with age, stature, and severity of the deformity.