■影响细胞壁多糖(EPS)的提取技术对于最大化其生物活性至关重要。本研究评估了超声波技术从念珠菌LG-8中提取抗氧化多糖,评估其对抗氧化活性的影响。
■优化了从G.candidumLG-8中超声提取EPS的方法(18分钟,pH7.0,40W/cm2,0.75MNaCl)。利用傅里叶变换红外光谱(FTIR)和扫描电镜(SEM)分析了LG-EPS1和LG-EPS3的ABTS清除效率和单糖组成。
■结果表明,超声处理可使LG-8细胞的ABTS自由基清除效率显着提高47%。在浓度为1mg/mL时,超声提取的LG-EPS1和LG-EPS3多糖表现出显着的ABTS自由基清除效率为26%和51%,分别。单糖组成分析鉴定了LG-EPS1中的甘露糖和葡萄糖,而LG-EPS3主要由甘露糖组成。FTIR光谱验证了多糖的存在,和SEM提供了LG-EPS1和LG-EPS3的纳米颗粒结构特征的视觉确认。
■这项研究不仅强调了超声波在多糖提取中的技术优点,而且还强调了G.candidumLG-8衍生多糖作为抗氧化剂利用的有价值的生物活性化合物的潜力。
UNASSIGNED: Extraction techniques that influence cell wall polysaccharides (EPS) is crucial for maximizing their bioactivity. This study evaluates ultrasound technology for extracting antioxidant polysaccharides from
Geotrichum candidum LG-8, assessing its impacton antioxidant activity.
UNASSIGNED: Ultrasound extraction of EPS from G. candidum LG-8 was optimized (18 min, pH 7.0, 40 W/cm2, 0.75 M NaCl). ABTS scavenging efficiency and monosaccharide composition of LG-EPS1 and LG-EPS3 were analyzed using Fourier-transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM).
UNASSIGNED: The Results showed that ultrasonic treatment markedly increased the ABTS radical scavenging efficiency of LG-8 cells by 47%. At a concentration of 1 mg/mL, the ultrasonically extracted LG-EPS1 and LG-EPS3 polysaccharides exhibited significant ABTS radical scavenging efficiencies of 26% and 51%, respectively. Monosaccharide composition analysis identified mannose and glucose in LG-EPS1, while LG-EPS3 was primarily composed of mannose. FTIR spectra verified the polysaccharides\' presence, and SEM provided visual confirmation of the nanoparticle structures characteristic of LG-EPS1 and LG-EPS3.
UNASSIGNED: This research not only underscores the technological merits of ultrasound in polysaccharide extraction but also highlights the potential of G. candidum LG-8 derived polysaccharides as valuable bioactive compounds for antioxidant utilization.