背景:Geotrichum是在世界各地不同生境中发现的真菌属。尽管地理学及其相关物种已被广泛地重新分类和分类学修订,它仍然是许多研究的目标。
结果:在这项研究中,在念珠菌和西维考菌之间进行了表型和分子遗传学比较。柳树琼脂被用作表型比较研究的生长培养基,其在两个温度(20-25和37°C)下进行。对于基因型比较,我们比较了18S,ITS,和两个物种的通用DNA条形码区域的28个S序列。结果揭示了用于真菌分离的新培养基的重要发现。两种菌落之间的表型变异,包括它们的形状,尺寸,纹理和生长速率,截然不同。两个物种的DNA序列表明,对于18S,该物种的成对同一性为99.9%,ITS为100%,28个S地区为99.6%。
结论:与通常看到的相反,结果表明,18S,ITS和28S未能区分该物种。在这项工作中报道了对柳树琼脂作为真菌培养基的性能的首次调查,证明了它的效率。此外,这是第一项通过表型和基因型分析比较G.candidum和G.silvicola的研究。
BACKGROUND: Geotrichum is a genus of fungi found in different habitats throughout the world. Although Geotrichum and its related species have been extensively reclassified and taxonomically revised, it is still the target for many researches.
RESULTS: In this
study, phenotypic and molecular genetics comparisons were performed between
Geotrichum candidum and
Geotrichum silvicola. Mitis Salivarius Agar was used as the growing medium for the phenotypic comparison
study, which was carried out at two temperatures (20-25 and 37 °C). For genotypic comparison, we compared the 18 S, ITS, and 28 S sequences of universal DNA barcode regions of both species. Important findings on the new culture media for fungal isolation were revealed by the results. The phenotypic variation between the two species\' colonies, including their shapes, sizes, textures and growth rates, were strikingly different. DNA sequences of both species showed that pairwise identities of the species were 99.9% for 18 S, 100% for ITS and 99.6% for 28 S regions.
CONCLUSIONS: Contrary to what is commonly seen, the results showed that 18 S, ITS and 28 S failed to discriminate the species. The first investigation into the performance of Mitis Salivarius Agar as a fungus culture medium is reported in this work, and proved its efficiency. Additionally, this is the first
study to compare G. candidum with G. silvicola by means of both phenotypic and genotypic analysis.