Abstract:
Identifying aflatoxin-detoxifying probiotics remains a significant challenge in mitigating the risks associated with aflatoxin contamination in crops. Biological detoxification is a popular technique that reduces mycotoxin hazards and garners consumer acceptance. Through multiple rounds of screening and validation tests, Geotrichum candidum XG1 demonstrated the ability to degrade aflatoxin B1 (AFB1) by 99-100%, exceeding the capabilities of mere adsorption mechanisms. Notably, the degradation efficiency was demonstrably influenced by the presence of copper and iron ions in the liquid medium, suggesting a potential role for proteases in the degradation process. Subsequent validation experiments with red pepper revealed an 83% reduction in AFB1 levels following fermentation with G. candidum XG1. Furthermore, mass spectrometry analysis confirmed the disruption of the AFB1 furan ring structure, leading to a subsequent reduction in its toxicity. Collectively, these findings establish G. candidum XG1 as a promising candidate for effective aflatoxin degradation, with potential applications within the food industry.
摘要:
识别黄曲霉毒素解毒益生菌仍然是减轻与作物中黄曲霉毒素污染相关的风险的重大挑战。生物解毒是一种流行的技术,可以减少霉菌毒素的危害并获得消费者的认可。通过多轮筛选和验证测试,念珠菌XG1显示出将黄曲霉毒素B1(AFB1)降解99-100%的能力,超出了单纯的吸附机制的能力。值得注意的是,降解效率明显受到液体介质中铜和铁离子的影响,提示蛋白酶在降解过程中的潜在作用。随后的红辣椒验证实验显示,用G.candidumXG1发酵后,AFB1水平降低了83%。此外,质谱分析证实了AFB1呋喃环结构的破坏,导致其毒性随后的降低。总的来说,这些发现确立了G.candidumXG1作为有效降解黄曲霉毒素的有希望的候选者,在食品工业中具有潜在的应用。