Abstract：
The Phylum Cressdnaviricota consists of a large number of circular Rep-encoding single-stranded (CRESS)-DNA viruses. Recently, metagenomic analyzes revealed their ubiquitous distribution in a diverse range of eukaryotes. Data relating to CRESS-DNA viruses in humans remains scarce. Our study investigated the presence and genetic diversity of CRESS-DNA viruses in human vaginal secretions. Vaginal swabs were collected from 28 women between 29 and 43 years old attending a fertility clinic in New York City. An exploratory metagenomic analysis was performed and detection of CRESS-DNA viruses was confirmed through analysis of near full-length sequences of the viral isolates. A phylogenetic tree was based on the REP open reading frame sequences of the CRESS-DNA virus genome. Eleven nearly complete CRESS-DNA viral genomes were identified in 16 (57.1%) women. There were no associations between the presence of these viruses and any demographic or clinical parameters. Phylogenetic analysis indicated that one of the sequences belonged to the genus Gemycircularvirus within the Genomoviridae family, while ten sequences represented previously unclassified species of CRESS-DNA viruses. Novel species of CRESS-DNA viruses are present in the vaginal tract of adult women. Although they be transient commensal agents, the potential clinical implications for their presence at this site cannot be dismissed.
摘要：
Cressdnaviricota由大量环状Rep编码单链（CRESS）DNA病毒组成。最近,宏基因组分析揭示了它们在各种真核生物中的普遍分布。与人类CRESS-DNA病毒有关的数据仍然很少。我们的研究调查了人阴道分泌物中CRESS-DNA病毒的存在和遗传多样性。从纽约市生育诊所就诊的28名29至43岁的女性中收集了阴道拭子。进行了探索性宏基因组分析，并通过分析病毒分离株的近全长序列证实了CRESS-DNA病毒的检测。系统发育树基于CRESS-DNA病毒基因组的REP开放阅读框序列。在16名（57.1％）女性中鉴定出11个几乎完整的CRESS-DNA病毒基因组。这些病毒的存在与任何人口统计学或临床参数之间没有关联。系统发育分析表明，其中一个序列属于基因组病毒科中的双环病毒属，而十个序列代表以前未分类的CRESS-DNA病毒物种。CRESS-DNA病毒的新物种存在于成年女性的阴道中。虽然他们是短暂的共生特工，他们在该站点的存在的潜在临床意义不能被忽略.
