PDF(Pubmed)
Abstract：
CONCLUSIONS: Genotyping-by-sequencing of 723 worldwide cucumber genetic resources revealed that cucumbers were dispersed eastward via at least three distinct routes, one to Southeast Asia and two from different directions to East Asia. The cucumber (Cucumis sativus) is an economically important vegetable crop cultivated and consumed worldwide. Despite its popularity, the manner in which cucumbers were dispersed from their origin in South Asia to the rest of the world, particularly to the east, remains a mystery due to the lack of written records. In this study, we performed genotyping-by-sequencing (GBS) on 723 worldwide cucumber accessions, mainly deposited in the Japanese National Agriculture and Food Research Organization (NARO) Genebank, to characterize their genetic diversity, relationships, and population structure. Analyses based on over 60,000 genome-wide single-nucleotide polymorphisms identified by GBS revealed clear genetic differentiation between Southeast and East Asian populations, suggesting that they reached their respective region independently, not progressively. A deeper investigation of the East Asian population identified two subpopulations with different fruit characteristics, supporting the traditional classification of East Asian cucumbers into two types thought to have been introduced by independent routes. Finally, we developed a core collection of 100 accessions representing at least 93.2% of the genetic diversity present in the entire collection. The genetic relationships and population structure, their associations with geographic distribution and phenotypic traits, and the core collection presented in this study are valuable resources for elucidating the dispersal history and promoting the efficient use and management of genetic resources for research and breeding in cucumber.
摘要：
结论：通过测序对全球723种黄瓜遗传资源进行基因分型显示，黄瓜通过至少三种不同的途径向东分散，一个到东南亚，两个从不同的方向到东亚。黄瓜（Cucumissativus）是世界范围内种植和消费的重要经济蔬菜作物。尽管它很受欢迎，黄瓜从南亚的起源分散到世界其他地方的方式，尤其是在东方，由于缺乏书面记录，仍然是一个谜。在这项研究中,我们对全球723个黄瓜品种进行了基因分型测序（GBS），主要存放在日本国家农业和粮食研究组织(NARO)Genebank，来表征它们的遗传多样性，关系,和人口结构。基于GBS鉴定的超过60,000个全基因组单核苷酸多态性的分析显示，东南亚和东亚人群之间存在明显的遗传差异。表明他们独立到达各自的地区，不是渐进的。对东亚种群进行了更深入的调查，发现了两个具有不同果实特征的亚种群，支持将东亚黄瓜传统分类为两种类型，这些类型被认为是通过独立路线引入的。最后,我们开发了一个包含100个种质的核心集合，代表了整个集合中至少93.2％的遗传多样性。遗传关系和种群结构，它们与地理分布和表型特征的关联，本研究提供的核心馆藏是阐明黄瓜传播历史和促进遗传资源有效利用和管理的宝贵资源，可用于黄瓜研究和育种。
