One of the main reasons for the decline in global freshwater biodiversity can be attributed to alterations in hydrological conditions resulting from dam construction. However, the majority of current research has focused on single or limited numbers of dams. Here, we carried out a seasonal fish survey, using environmental DNA (eDNA) method, on the Wujiang River mainstream (Tributaries of the Yangtze River, China) to investigate the impact of large-scale cascade hydropower development on changes in fish diversity patterns. eDNA survey revealed that native fish species have decreased in contrast to alien fish. There was also a shift in fish community structure, with declines of the dominant rheophilic fish species, an increase of the small-size fish species, and homogenization of species composition across reservoirs. Additionally, environmental factors, such as temperature, dissolved oxygen and reservoir age, had a significant effect on fish community diversity. This study provides basic information for the evaluation of the impact of cascade developments on fish diversity patterns.

Species of the catfish genus Rineloricaria are common in the Paraíba do Sul River basin, in southeastern Brazil; here we present a revision of the taxonomic diversity and geographic distribution of the species of the genus inhabiting the basin, based on novel morphologic and molecular data. Five species delimitation methods based on cytochrome C oxidase subunit 1 nucleotide sequences yielded comparable molecular operational taxonomic units. The automatic barcode gap discovery, assemble species by automatic partitioning, barcode index number, and Bayesian implementation of the Poisson tree process methods supported the recognition of five evolutionary lineages. These taxonomic units were assigned to the previously described Rineloricaria nigricauda, Rineloricaria steindachneri, Rineloricaria zawadzkii, and Rineloricaria nudipectoris, and an additional undescribed species. R. zawadzkii was further divided into two intraspecific geographically structured lineages using the generalized mixed Yule coalescent delimitation method. A maximum likelihood phylogenetic analysis revealed that the five lineages from the Paraíba do Sul have closer relationships to different species from southern and southeastern Brazil (Ribeira de Iguape, Lagoa dos Patos, Uruguay, Paraguay, and Parana river basins) than to each other. Based on the analysis of lectotypes, recently collected material, and specimens from ichthyological collections, the poorly described R. nigricauda and R. steindachneri are redescribed following current descriptive standards. The undescribed species from the middle and upper Paraíba do Sul River basin is formally described. The description of a new species, along with the description of species boundaries in R. nigricauda and R. steindachneri, contributes to the knowledge of the ichthyofauna of the Paraíba do Sul River basin and adjacent coastal drainages of southeastern Brazil. An identification key for the species of Rineloricaria occurring in the Paraíba do Sul River basin is provided.

Plateau river ecosystems are often highly vulnerable and responsive to environmental change. The driving mechanism of fish diversity and community assembly in plateau rivers under changing environments presents a significant complexity to the interdisciplinary study of ecology and environment. This study integrated molecular biological techniques and mathematical models to identify the mechanisms influencing spatial heterogeneity of freshwater fish diversity and driving fish community assembly in plateau rivers. By utilizing environmental-DNA metabarcoding and the null model, this study revealed the impact of the stochastic process on fish diversity variations and community assembly in the Huangshui Plateau River of the Yellow River Basin (YRB) in China. This research identified 30 operational taxonomic units (OTUs), which correspond to 20 different fish species. The findings of this study revealed that the fish α-diversity in the upstream region of Xining is significantly higher than in the middle-lower reach (Shannon index: P = 0.017 and Simpson: P = 0.035). This pattern was not found to be related to any other environmental factors besides altitude (P = 0.023) that we measured. Further, the study indicated that the assembly of fish communities in the Huangshui River primarily depends on stochastic ecological processes. These findings suggested that elevation was not the primary factor impacting the biodiversity patterns of fish in plateau rivers. In plateau rivers, spatial heterogeneity of fish community on elevation is mainly determined by stochastic processes under habitat fragmentation, rather than any other physicochemical environmental factors. The limitations of connectivity in the downstream channel of the river could be taken the mainly responsibility for stochastic processes of fish community in Huangshui River. Incorporating ecological processes in the eDNA approach holds great potential for future monitoring and evaluation of fish biodiversity and community assembly in plateau rivers.

The concept of \"state fishes of India\" highlights the importance and significance of the prioritized fish species distributed across various states within the country. This review article systematically documents the current status of state fishes from various perspectives, identifies the prevailing knowledge gaps, and also highlights the issues and strategic plans essential for the conservation and sustainable utilization of these valuable genetic resources. A total of 8357 publications were checked for the consolidated information on state fish species, and the appropriate items were selected under eight categories: biology, physiology and nutrition, aquaculture, habitat and environmental parameters, genetics and biotechnology, harvest and postharvest, fish health management, and others. The synthesized information was used to present the current status of research and development on state fish species. The knowledge gaps that are to be addressed are also depicted under the perspectives of fisheries management and conservation aquaculture. Based on the findings, strategic plans for the targeted conservation programmes are proposed and discussed under various in situ and ex situ conservation measures. Further, the departmental processes involved in the declaration, the importance of stakeholder involvement, namely, local communities and policymakers, in fostering effective conservation measures, and planning for utilization of these valuable fish genetic resources are also indicated.

This study investigates the fish assemblage in a temporarily-closed sandbar estuary along India\'s west coast, across different zones and seasons. Moderate species diversity (54 species), low species abundance, varying fish diversity from lower to upper estuarine gradient (higher counts in lower zones, and decreasing from middle to upper zones), and higher prevalence of marine migrants (estuarine use) and piscivorous species (feeding mode) were the significant characteristics of the estuary. Distinct spatial, seasonal, and estuarine mouth state-based variations were recorded in the estuary, based on diversity indices and count of taxa. Peak diversity and abundance were noted during the pre-monsoon, and during the open mouth state of the estuary. The entire estuarine gradient is anthropogenically impacted by a multitude of stressors, except at the estuarine mouth region, which is favored by marine connectivity. The fish assemblage structure in Poonthura estuary (PE) has thus far remained unaffected by anthropogenic disturbances. Nevertheless, growth and nursery function of the estuary have been significantly compromised. The predominance of juvenile fish in the ecosystem, and prevailing anthropogenic stressors from point and non-point sources underscore the necessity for long-term maintenance of the population of coastal species, as well as a need for developing and implementing urgent management strategies for this fragile ecosystem.

With 76 currently valid species, the bushynose catfish genus Ancistrus is the fourth most species-rich catfish genus, yet Ancistrus diversity remains underestimated, with many species still undescribed. This is especially true of the Peruvian Andean headwaters of the Amazon, which are rich in unnamed Ancistrus species but have received little recent taxonomic attention. We describe a distinctively striped new Ancistrus species from tributaries of the Palcazú River, in the Pachitea-Ucayali-Amazonas drainage basin. The new species differs from all congeners by having black, vermiculated lines covering the head and two to four distinct black, parallel, lateral body stripes from head to caudal fin (vs. body uniformly colored or with dark or light spots or blotches over head and body, or black vermiculate lines on flanks). The new species is the fifth valid species of Ancistrus described from the rich Ucayali River ichthyofauna. It has previously been recognized in the aquarium fish trade as L267.

To monitor the changes in fish biodiversity and to elucidate the factors responsible for these changes, the landings composition (LC) over the past 30 years in the Nile-Delta lakes was quantitatively analyzed. The LC data indicates a shift in target species towards demersal opportunistic species. A significant difference (P < 0.001) between two main intervals highlighted in both PERMANOVA and PCA plot; the first interval (1991-2002) is dominated by Tilapia and rare species, while the second interval (2003-2020) is dominated by the opportunistic catfish and mullet species. Noticeable declines in species richness and landings of rare species were observed, where rare taxa either have been overexploited or they may be positively affected by increasing pollution levels than do other dominated species such as Tilapia. In contrast, opportunistic fish species such as catfish and mullet, have flourished in such polluted water due to their ability to tolerate seasonal pollution-related hypoxia.

Freshwater ecosystems are under great threat from humans, among which habitat heterogeneity is the most obvious, being one of the important reasons for the decline of fish diversity. This phenomenon is particularly prominent in the Wujiang River, where the continuous rapids of the mainstream have been divided into 12 mutually isolated sections by 11 cascade hydropower reservoirs. Based on the fact that conventional survey methods are more harmful to the ecological environment, the efficient and noninvasive environmental DNA metabarcoding (eDNA) approach was used in this study to conduct an aquatic ecological survey of the 12 river sections of the mainstream of the Wujiang River. A total of 2299 operational taxonomic units (OTUs) were obtained, corresponding to 97 species, including four nationally protected fish species and 12 alien species. The results indicate that the fish community structure of the Wujiang River mainstream, which was originally dominated by rheophilic fish species, has been changed. And there are differences in fish species diversity and species composition among the reservoir areas of the mainstream of the Wujiang River. The fish species in the area have gradually declined under the influence of anthropogenic factors such as terraced hydropower and overfishing. The fish populations consequently have demonstrated a tendency to be species miniaturized, and the indigenous fish are severely threatened. In addition, the fish composition monitored by the eDNA approach was found to be close to the fish composition of historical information on the Wujiang River, indicating that eDNA approach may be used as a complementary tool to conventional methods in this basin.

Traditionally, species of fish are identified based on morphological characteristics. Although these taxonomic descriptions are essential, there are cases where the morphological characters distinguishing these species show marginal differences. For instance, in the Poonch River in the Himalayas, there are 21 species, out of which some are morphologically similar, and the taxonomic distinction between these species is unclear. Therefore, in this study, we used sequences from two mitochondrial genes, Cytochrome b (Cyt b) and a larger ribosomal subunit (16S rRNA), as well as the morphological analysis to address any taxonomic ambiguities among the six fish species. Maximum Likelihood results revealed that all the species were clustered according to their families and genera. The phenotypic analysis also supported this statement, as all the species of different genera like Schizothorax, Tor, Garra, Traqilabeo, and Glyptothorax are grouped in their particular cluster, it shows that species of a separate class share a mutual morphological characteristic. While genetic analyses of these species suggest nucleotide diversity (p) and haplotype diversity, with Hd values as 0.644 for Cyt b and 0.899 for 16S rRNA, confirming the rich genetic diversity in the river. Overall, we recommend that the integrative approach in delimiting the fish species is more effective than the individual one and can be used to rapidly diagnose a species and understand the evolutionary relationship between the species.

Tanguar Haor (TH), an ecologically critical area (ECA) and a Ramsar site of worldwide significance, is an essential wetland ecosystem for the Bangladesh\'s economic, ecological, social, and cultural aspects. Fish, aquatic plants, amphibians, reptiles, birds, and mammals are notable among the floral and faunal compositions found in this haor. Unfortunately, unsustainable exploitation of its natural resources poses a serious threat to the TH ecosystem. Therefore, the broad objective of this study was to review the status of fish biodiversity along with the driving factors of biodiversity loss and the management issues of the TH ecosystem. A total of 143 species of fishes (137 indigenous and 6 exotic) under 35 families, and 12 orders were documented during the last two decades. Species diversity of the haor has been changed over time due to the effects of climatic, anthropogenic, socioeconomic, and policy related drivers. Furthermore, high dependency on fisheries resources, poverty, and the lack of empowerment to manage the TH fishery were responsible for fish diversity decline. Therefore, ecosystem based co-management through active participation of local community, establishment of balanced fishing tactics, and strengthening alternative livelihoods for highly depended poor harvesters are strongly recommended for the proper management of this valued wetland ecosystem. Furthermore, this review proposes immediate and useful conservation initiatives for the studied wetlands, including comprehensive stock assessment, establishment of gene banks and fish sanctuaries, a combination of input and output control, and regulation with the ECA and RAMSAR guidelines.