PDF(Pubmed)
Abstract：
Traditionally, species of fish are identified based on morphological characteristics. Although these taxonomic descriptions are essential, there are cases where the morphological characters distinguishing these species show marginal differences. For instance, in the Poonch River in the Himalayas, there are 21 species, out of which some are morphologically similar, and the taxonomic distinction between these species is unclear. Therefore, in this study, we used sequences from two mitochondrial genes, Cytochrome b (Cyt b) and a larger ribosomal subunit (16S rRNA), as well as the morphological analysis to address any taxonomic ambiguities among the six fish species. Maximum Likelihood results revealed that all the species were clustered according to their families and genera. The phenotypic analysis also supported this statement, as all the species of different genera like Schizothorax, Tor, Garra, Traqilabeo, and Glyptothorax are grouped in their particular cluster, it shows that species of a separate class share a mutual morphological characteristic. While genetic analyses of these species suggest nucleotide diversity (p) and haplotype diversity, with Hd values as 0.644 for Cyt b and 0.899 for 16S rRNA, confirming the rich genetic diversity in the river. Overall, we recommend that the integrative approach in delimiting the fish species is more effective than the individual one and can be used to rapidly diagnose a species and understand the evolutionary relationship between the species.
摘要：
传统上,根据形态特征鉴定鱼类。尽管这些分类描述是必不可少的，在某些情况下，区分这些物种的形态特征显示出边际差异。例如,在喜马拉雅山的Poonch河里,有21种，其中一些在形态上相似，这些物种之间的分类区别尚不清楚。因此,在这项研究中，我们使用了两个线粒体基因的序列，细胞色素b（Cytb）和一个更大的核糖体亚基（16SrRNA），以及形态学分析，以解决六种鱼类之间的任何分类歧义。最大似然结果显示，所有物种都根据其科和属进行聚类。表型分析也支持这一说法，就像所有不同属的物种一样，Tor,Garra,Traqilabeo,和眼胸被分组在它们的特定集群中，它表明，一个单独类别的物种具有共同的形态特征。虽然这些物种的遗传分析表明核苷酸多样性（p）和单倍型多样性，Cytb的Hd值为0.644，16SrRNA的Hd值为0.899，证实了河流中丰富的遗传多样性。总的来说,我们建议在界定鱼类物种时采用综合方法比单独的方法更有效，并且可以用于快速诊断物种并了解物种之间的进化关系。
