抗菌素耐药性是对公众健康的威胁。抗生素抗性金黄色葡萄球菌的出现代表了实施预防措施的优先事项。目的是在人体中分离金黄色葡萄球菌,动物,和动物保健环境,并表征这些分离株中抗生素耐药性的基因型和表型特征。我们从工作人员身上分离出金黄色葡萄球菌,动物,和兽医医院的环境,并确定了他们的抗菌素耐药性。采集了20个人的样本,13只动物,14个表面,8手机,和7名兽医听诊器使用无菌拭子。通过在甘露醇盐琼脂上培养分离金黄色葡萄球菌,并通过革兰氏染色和过氧化氢酶试验进行初步鉴定。随后,我们进行了聚合酶链反应以确认物种并调查其抗菌素耐药基因型.使用圆盘扩散技术确定抗性分离株的表型特征。从5/20人中回收了10株金黄色葡萄球菌分离株(25%),它也从2/13的动物(15.38%)中恢复,包括一只狗和一只猫,和1/14的表面(7.14%)。在猫科动物中鉴定了对苯唑西林敏感的mecA阳性金黄色葡萄球菌表型。大多数分离株携带至少两种不同抗菌类别的抗性基因,90%(9/10)的基因是blaZ,10%(1/10)的基因是mecA,20%(2/10)呈现tet38,10%(1/10)呈现tetM,90%(9/10)呈现norA,50%(5/10)呈现正常温度,10%(1/10)呈现ermA,60%(6/10)呈现ermB。在抗生素方面,在所有的分离物中都发现了对青霉素的抗性,80%(8/10)的人对红霉素耐药,所有分离株对红霉素的抗性均表现为红霉素诱导的克林霉素抗性。由于种间传播的风险,兽医医院的抗菌素耐药性需要注意,寄居于伴侣动物和人类的细菌之间的基因转移,会使抗菌治疗变得困难。
Antimicrobial resistance is a threat to public health. The emergence of antibiotic-resistant Staphylococcus aureus represents a priority for the implementation of preventive measures. The objective was to isolate S. aureus in humans, animals, and animal health care environment, and to characterize the genotypic and phenotypic profile of antimicrobial resistance in these isolates. We isolated S. aureus from staff, animals, and environment of a veterinary hospital, and identified their antimicrobial resistance profiles. Samples were collected from 20 humans, 13 animals, 14 surfaces, 8 mobile phones, and 7 veterinarians\' stethoscopes by using sterile swabs. S. aureus was isolated by culturing on mannitol salt agar and preliminary identification was done by Gram staining and catalase test. Subsequently, a polymerase chain reaction was performed for species confirmation and investigating their antimicrobial-resistant genotypic profiles. Phenotypic profiles of resistant isolates were determined using the disk-diffusion technique. Ten S. aureus isolates were recovered from 5/20 humans (25%), it was also recovered from 2/13 animals (15.38%), including 1 dog and 1 cat, and from 1/14 of surfaces (7.14%). The oxacillin-susceptible mecA-positive Staphylococcus aureus phenotype was identified in a feline. Most of the isolates carried at least two resistance genes of different antimicrobial classes, with 90% (9/10) presenting the gene blaZ, with 10% (1/10) presenting the gene mecA, 20% (2/10) presenting tet38, 10% (1/10) presenting tetM, 90% (9/10) presenting norA, 50% (5/10) presenting norC, 10% (1/10) presenting ermA, and 60% (6/10) presenting ermB. In antibiograms, resistance to penicillin was identified in all the isolates, resistance to erythromycin was identified in 80% (8/10), and all the isolate\'s resistance to erythromycin presented erythromycin-induced resistance to clindamycin. Antimicrobial resistance in the veterinary hospital requires attention due to the risk of interspecies transmission, gene transfer between bacteria that colonize companion animals and humans and, can make antimicrobial therapy difficult.