L-色氨酸已被用作猪和家禽的饲料添加剂以及人类的营养补充剂。然而,据报道,L-色氨酸中的一些杂质是嗜酸性粒细胞增多-肌痛综合征的致病成分。因此,从安全的角度来看,重要的是分析肉类样品中的这些杂质。本研究旨在开发一种使用LC-MS/MS同时检测肉制品中L-色氨酸杂质的分析方法。在各种杂质中,(S)-2-氨基-3-(5-羟基-1H-吲哚-3-基)丙酸(5-羟色氨酸)(HTP)的检测方法,1-甲基-1,2,3,4-四氢-β-咔啉-3-羧酸(MTCA),3a-羟基-1,2,3,3a,8,8a-六氢吡咯并-[2,3-b]-吲哚-2-羧酸(PIC),并开发了1,1'-亚乙基二色氨酸(EBT)和2-(3-吲哚甲基)-L-色氨酸(IMT)。所开发的方法可以在5分钟内同时测定这四种杂质。没有观察到来自矩阵的干扰,并且该方法对每种分析物显示出良好的灵敏度。方法检出限和定量限在肉基质中低于11.2和35.7μg/kg,分别。
L-tryptophan has been used as a feed additive for swine and poultry and as a nutrient supplement for humans. However, some impurities in L-tryptophan have been reported as causative components of eosinophilia-myalgia syndrome. Therefore, from a safety perspective, it is important to analyze meat samples for these impurities. This study aims to develop an analytical method for the simultaneous detection of L-tryptophan impurities in meat products using LC-MS/MS. Among the various impurities, detection methods for (S)-2-amino-3-(5-hydroxy-1H-indol-3-yl)propanoic acid (5-hydroxytryptophan) (HTP), 1-methyl-1,2,3,4-tetrahydro-β-carboline-3-carboxylic acid (MTCA), 3a-hydroxy-1,2,3,3a,8,8a-hexahydropyrrolo-[2,3-b]-indole-2-carboxylic acid (PIC), and 1,1\'-ethylidenebistryptophan (EBT) and 2-(3-indoylmethyl)-L-tryptophan (IMT) were developed. The developed method allowed simultaneous determination of these four impurities in 5 min. No interferences from the matrix were observed, and the method showed good sensitivity to each analyte. The method detection limit and limit of quantification in meat matrices were below 11.2 and 35.7 μg/kg, respectively.