{Reference Type}: Journal Article
{Title}: Safety evaluation of dried L-tryptophan fermentation product in Sprague-Dawley rats.
{Author}: Ko SM;Park JE;Kim SH;Park MK;Seol JK;Heo IK;Shin YU;Kim SY;Kim YH;Son WC;
{Journal}: Toxicol Rep
{Volume}: 9
{Issue}: 0
{Year}: 2022
暂无{DOI}: 10.1016/j.toxrep.2022.05.005
{Abstract}: The subchronic toxicity of oral L-tryptophan produced by fermentation with metabolically engineered Corynebacterium glutamicum was evaluated in Sprague-Dawley rats. Doses of 0, 500, 1000, and 2000 mg/kg/day were administered to groups of 10 male and 10 female rats for 90 days. For the groups administered 0 and 2000 mg/kg/day, an additional 5 male and 5 female rats were tested as a recovery group. No adverse effects associated with the test substance were observed in all rats during the 90-day administration of the product, irrespective of dose, and at 4 weeks of recovery at dosages of 0 and 2000 mg/kg/day. Furthermore, histochemical and immunohistochemical analyses for L-tryptophan-associated eosinophilia-myalgia syndrome (EMS) did not reveal significant changes in both sexes of groups administered 0 or 2000 mg/kg/day. Based on these results, it could be concluded that there were no significant adverse effects related to the test substance in all animals; therefore, dried L-tryptophan fermentation product can be used as feed additive material.