UNASSIGNED:自体培养的表皮(CE)是克服供体部位缺陷以治疗大面积烧伤的有效方法。然而,自体CE的生产需要3-4周,这阻止了它在严重烧伤的危及生命期间的使用。相比之下,同种异体CE可以提前准备并用作伤口敷料,在施用部位释放几种刺激受体细胞活性的生长因子。通过在受控的温度和湿度条件下干燥CE直到完全除去所有的水并且不存在活细胞来制备干燥的CE。干燥的CE可加速鼠类皮肤缺损模型中的伤口愈合,并且可能是一种新的治疗策略。然而,干燥的CE安全性和有效性尚未在大型动物模型中进行研究。因此,我们使用小型猪模型研究了人类干燥CE在伤口愈合中的安全性和有效性。
未经鉴定:使用Green方法从供体角质形成细胞制造人CE。三种类型的CE(新鲜,冷冻保存,和干的)准备好了,并且在体外证实了每种CE促进角质形成细胞增殖的能力。将三种CE的提取物添加到接种在12孔板中的角质形成细胞中,并使用WST-8测定评估细胞增殖7天。接下来,我们在小型猪的背部制备了部分厚度的皮肤缺损,并应用了三种类型的人类CE来评估伤口愈合的促进作用。在第4天和第7天,收集苏木精-伊红样本,阿赞,和抗CD31染色以评估上皮形成,肉芽组织,和毛细管形成。
UNASSIGNED:与对照组相比,含有干燥CE提取物的条件培养基显著增强角质形成细胞增殖(P<0.05)。体内实验表明,人类干燥的CE在第7天显着加速上皮形成,与新鲜CE相同。与对照组比较(P<0.05)。三个CE组类似地影响肉芽形成和新血管形成。
未经证实:干燥的CE在猪部分厚度皮肤缺损模型中加速上皮化,这表明它可能是一种有效的烧伤治疗方法。需要进行长期随访的临床研究,以评估CE在临床中的适用性。
UNASSIGNED: Autologous cultured epidermis (CE) is an effective approach for overcoming the deficiency of donor sites to treat extensive burns. However, the production of autologous CE takes 3-4 weeks, which prevents its use during the life-threatening period of severe burns. In contrast, allogeneic CE can be prepared in advance and used as a wound dressing, releasing several growth factors stimulating the activity of recipient cells at the application site. Dried CE is prepared by drying CEs under controlled temperature and humidity conditions until all the water is completely removed and no viable cells are present. Dried CE accelerates wound healing in a murine skin defect model and is potentially a new therapeutic strategy. However, the dried CE safety and efficacy have not yet been studied in large animal models. Therefore, we studied the safety and efficacy of human-dried CE in wound healing using a miniature swine model.
UNASSIGNED: Human CE was manufactured using Green\'s method from donor keratinocytes. Three types of CEs (Fresh, Cryopreserved, and Dried) were prepared, and the ability of each CE to promote keratinocyte proliferation was confirmed in vitro. Extracts of the three CEs were added to keratinocytes seeded in 12-well plates, and cell proliferation was evaluated using the WST-8 assay for 7 days. Next, we prepared a partial-thickness skin defect on the back of a miniature swine and applied three types of human CE to evaluate wound healing promotion. On days 4 and 7, the specimens were harvested for hematoxylin-eosin, AZAN, and anti-CD31 staining to assess epithelialization, granulation tissue, and capillary formation.
UNASSIGNED: The conditioned medium containing dried CE extract significantly enhanced keratinocyte proliferation compared to the control group (P < 0.05). In vivo experiments revealed that human-dried CE significantly accelerated epithelialization at day 7 to the same extent as fresh CE, compared to the control group (P < 0.05). The three CE groups similarly affected granulation formation and neovascularization.
UNASSIGNED: Dried CE accelerated epithelialization in a porcine partial-thickness skin defect model, suggesting that it may be an effective burn treatment alternative. A clinical study with a long-term follow-up is needed to assess the applicability of CEs in clinics.