■鉴于对人类健康的重大影响,必须开发新的糖尿病伤口治疗方法,这是糖尿病的普遍和严重的并发症。糖尿病伤口微环境具有高水平的活性氧(ROS)以及促炎和抗炎细胞/因子之间的失衡,这阻碍了慢性伤口的愈合。本研究旨在开发含有姜黄素和银纳米颗粒(AgNPs)的聚(L-乳酸)(PLLA)纳米纤维膜,定义为PLLA/C/Ag,用于糖尿病伤口愈合。
■PLLA/C/Ag通过空气喷射纺丝方法制造。通过包括傅里叶变换红外光谱在内的各种技术对膜进行了制备和表征,水接触角的测量,X射线光电子能谱,X射线衍射,扫描电子显微镜,姜黄素和Ag+的体外释放评估,机械强度测试,灵活性,吸水性和生物降解性。此外,抗氧化剂,在体外评估膜的抗菌和抗炎特性,并且使用糖尿病小鼠在体内测试了膜愈合伤口的能力。
■通过空气喷射纺丝制备具有均匀纤维尺寸的松散亲水性纳米纤维膜。所述膜能够有效和持续地释放姜黄素。更重要的是,抗菌AgNPs成功地从AgNO3原位还原。AgNPs的掺入赋予了膜优越的抗菌活性,姜黄素和AgNPs的生物活性给予膜有效的ROS清除和免疫调节作用,保护细胞免受氧化损伤并减少炎症。动物研究的进一步结果表明,PLLA/C/Ag膜具有最有效的伤口愈合特性,这是通过刺激血管生成和胶原蛋白沉积以及抑制炎症来实现的。
■在这项研究中,我们成功地制造了具有抗氧化剂特性的PLLA/C/Ag膜,抗菌剂和抗炎剂,可以帮助伤口愈合。调节伤口炎症,这些新的PLLA/C/Ag膜作为一种新型敷料,可增强糖尿病伤口的愈合。
UNASSIGNED: Given the significant impact on human health, it is imperative to develop novel treatment approaches for diabetic wounds, which are prevalent and serious complications of diabetes. The diabetic wound microenvironment has a high level of reactive oxygen species (ROS) and an imbalance between proinflammatory and anti-inflammatory cells/factors, which hamper the healing of chronic wounds. This study aimed to develop poly(L-lactic acid) (PLLA) nanofibrous membranes incorporating curcumin and silver nanoparticles (AgNPs), defined as PLLA/C/Ag, for diabetic wound healing.
UNASSIGNED: PLLA/C/Ag were fabricated via an air-jet spinning approach. The membranes underwent preparation and characterization through various techniques including Fourier-transform infrared spectroscopy, measurement of water contact angle, X-ray photoelectron spectroscopy, X-ray diffraction, scanning electron microscopy, assessment of in vitro release of curcumin and Ag+, testing of mechanical strength, flexibility, water absorption and biodegradability. In addition, the antioxidant, antibacterial and anti-inflammatory properties of the membranes were evaluated in vitro, and the ability of the membranes to heal wounds was tested in vivo using diabetic mice.
UNASSIGNED: Loose hydrophilic nanofibrous membranes with uniform fibre sizes were prepared through air-jet spinning. The membranes enabled the efficient and sustained release of curcumin. More importantly, antibacterial AgNPs were successfully reduced in situ from AgNO3. The incorporation of AgNPs endowed the membrane with superior antibacterial activity, and the bioactivities of curcumin and the AgNPs gave the membrane efficient ROS scavenging and immunomodulatory effects, which protected cells from oxidative damage and reduced inflammation. Further results from animal studies indicated that the PLLA/C/Ag membranes had the most efficient wound healing properties, which were achieved by stimulating angiogenesis and collagen deposition and inhibiting inflammation.
UNASSIGNED: In this research, we successfully fabricated PLLA/C/Ag membranes that possess properties of antioxidants, antibacterial agents and anti-inflammatory agents, which can aid in the process of wound healing. Modulating wound inflammation, these new PLLA/C/Ag membranes serve as a novel dressing to enhance the healing of diabetic wounds.