Abstract：
Diabetic wounds require a multifactorial approach because several factors are involved in its occurrence. Herein we investigated whether transplantation of hyaluronic acid (HA) in combination with menstrual blood derived stem cells (MenSCs) could promote healing in diabetic rats. Thirty days after induction of diabetes, sixty animals were randomly planned into four equal groups: the untreated group, HA group, MenSC group, and HA+MenSC group. Sampling was done for histological, molecular, and tensiometrical assessments. Our results indicated that the wound contraction rate, volumes of new epidermis and dermis, collagen density, as well as tensiometrical parameter were considerably increased in the treatment groups compared to the untreated group and these changes were more obvious in the HA+MenSC ones. In addition, the expression levels of TGF-β and VEGF genes were significantly upregulated in treatment groups in comparison with the untreated group and were greater in the HA+MenSC group. This is while expression levels of TNF-α and IL-1β genes were more considerably downregulated in the HA+MenSC group than the other groups. We concluded that the combined use of HA and MenSCs has more effects on diabetic wound healing.
摘要：
糖尿病伤口需要多因素方法，因为其发生涉及多个因素。本文研究了透明质酸（HA）与经血来源的干细胞（MenSCs）联合移植是否可以促进糖尿病大鼠的愈合。诱导糖尿病30天后，将60只动物随机分为4组：未治疗组，HA组,MenSC组,和HA+MenSC组。进行组织学取样，分子，和张力计评估。我们的结果表明，伤口收缩率，新表皮和真皮的体积，胶原蛋白密度，与未治疗组相比，治疗组的张力计参数显着增加，而这些变化在HAMenSC组中更为明显。此外,与未治疗组相比,治疗组TGF-β和VEGF基因的表达水平显著上调,而HA+MenSC组则更高.这是在HA+MenSC组中,TNF-α和IL-1β基因的表达水平比其他组显著下调的同时。我们得出结论，HA和MenSCs的联合使用对糖尿病伤口愈合有更多的影响。
