在过去的十年中,已经报道了越来越多的由棒状杆菌引起的不同临床感染。这项研究的目的是评估抗生素耐药率,生物膜形成能力,并研究棒状杆菌的抗群体感应(抗QS)活性,根据培养物的生长类型分为三组(纯,与另一种致病细菌和多微生物生长)。总共240个棒状杆菌属。在2021年6月至2022年6月之间,从不同的临床标本中分离出的菌株被分为三组:纯,纯,与另一种病原体和多微生物分离,根据他们在文化中的生长模式。通过基质辅助激光解吸电离飞行时间质谱(MALDI-TOFMS)Biotyper(Bruker,德国)在外部中心。通过圆盘扩散法测定抗生素敏感性,对于万古霉素肉汤,使用微量稀释法。结果根据EUCAST建议进行解释。定量测定分离物的生物膜形成特性。提取了17种具有强生物膜形成的分离株的生物活性成分,并使用紫色杆菌ATCC12472菌株通过琼脂扩散法测定了抗QS活性,然后定量测量了紫罗兰素色素的产生。在240个棒状杆菌属中。隔离物,138(58%)是纯的,52(22%)与另一种病原体分离,50(20%)是多微生物感染的一部分。在隔离物中,140人被鉴定为纹状体梭菌,34为淀粉样C.colatum和24为游离棒状杆菌。当根据组分析棒状杆菌分离株的抗生素耐药率时,发现多重微生物组对利福平和四环素类抗生素的耐药率在统计学上显著低于其他组.对青霉素的耐药率,克林霉素,环丙沙星,莫西沙星,利福平,四环素和利奈唑胺占96.7%,88.3%,86.3%,73.8%,62.5%,59.2%和0.8%,分别。虽然所有分离株都对万古霉素敏感,利奈唑胺耐药在两个非洲衣原体分离株中检测到。当分析生物膜形成能力时,观察到87(36.3%)分离株形成了生物膜。微生物生长组中分离物的生物膜形成率低于其他两组。研究了17种具有强生物膜形成的分离株的抗QS活性,并且在使用紫菜的QS研究中,没有发现测试的棒状杆菌提取物具有抗QS活性(抑制紫罗兰素色素产生而不抑制细菌生长)。而五种分离提取物具有抗菌活性(抑制细菌生长)。具有抗菌活性的细菌提取物中的四种属于淀粉芽孢杆菌,一种属于C.afermentans。总之,当分析抗生素耐药率和生物膜形成率时,与另一种病原体一起培养的棒状杆菌表现出与纯培养的棒状杆菌相似的特征。因此,人们认为,与另一种病原体一起生长的棒状杆菌不应被忽视。此外,一些棒状杆菌提取物的抗菌作用表明,更多的QS研究应该用微生物进行。
An increasing number of different clinical infections caused by Corynebacteria have been reported in the last decade. The aim of this study was to evaluate the antibiotic resistance rates, biofilm formation capacities and to investigate the \'\'anti-quorum-sensing (anti-QS)\'\' activities of corynebacteria, which were divided into three groups according to the type of growth in culture (pure, with another pathogenic bacterium and polymicrobial growth). In total 240 Corynebacterium spp. isolates from different clinical specimens sent to the medical microbiology laboratories of Düzce University Faculty of Medicine Hospital and Başakşehir Çam and Sakura City Hospital between June 2021 and June 2022 were classified into three groups: pure, isolated with another pathogen and polymicrobial, according to their growth patterns in culture. Bacteria were identified by matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) Biotyper (Bruker, Germany) at an external centre. Antibiotic susceptibilities were determined by disc diffusion method and for vancomycin broth microdilution method was used. Results were interpreted according to EUCAST recommendations. The biofilm-forming properties of the isolates were determined quantitatively. Bioactive components of 17 isolates with strong biofilm formation were extracted and anti-QS activity was determined by agar diffusion method using Chromobacterium violaceum ATCC 12472 strain and then violacein pigment production was measured quantitatively. Of the 240 Corynebacterium spp. isolates, 138 (58%) were pure, 52 (22%) were isolated with another pathogen and 50 (20%) were part of a polymicrobial infection. Of the isolates, 140 were identified as C.striatum, 34 as C.amycolatum and 24 as Corynebacterium afermentans. When the antibiotic resistance rates of the Corynebacterium isolates were analysed according to the groups, the resistance rates to rifampicin and tetracycline antibiotics were found to be statistically significantly lower in the polymicrobial group than in the other groups. The resistance rates to penicillin, clindamycin, ciprofloxacin, moxifloxacin, rifampicin, tetracycline and linezolid were 96.7%, 88.3%, 86.3%, 73.8%, 62.5%, 59.2% and 0.8%, respectively. While all isolates were susceptible to vancomycin, linezolid resistance was detected in two C.afermentans isolates. When the biofilm formation ability was analysed, it was observed that 87 (36.3%) isolates formed biofilm. The biofilm formation rate of the isolates in the polymicrobial growth group was lower than the other two groups. The anti-QS activity of 17 isolates with strong biofilm formation was investigated and none of the Corynebacterium extracts tested were found to have anti-QS activity (inhibition of violacein pigment production without inhibiting bacterial growth) in the QS study with C.violaceum, whereas five isolate extracts had antibacterial activity (inhibition of bacterial growth). Four of the bacterial extracts with antimicrobial activity belonged to C.amycolatum and one to C.afermentans. In conclusion, when both antibiotic resistance rates and biofilm formation rates were analysed, the corynebacteria growing in culture with another pathogen showed similar characteristics to the corynebacteria growing as a pure culture. Therefore, it was thought that corynebacteria growing with another pathogen should not be ignored. In addition, the antimicrobial effects of some corynebacterial extracts suggested that more QS studies should be carried out with microbiota bacteria.