这项研究研究了铜纳米颗粒(CuNPs)对细菌嗜水气单胞菌的体外抗菌和驱虫作用,单基因Dactylogyrusminutus,Dactylogyrus扩展名,Cyprini陀螺,和死体Schyzocotyleacheilognathi,以及它们对鲤鱼的毒性。在体外抗菌试验中,进行了抑制区方法和最低抑制浓度(MIC)。为了确定单系寄生虫死亡率的时间和疗效,将寄生虫暴露于浓度为20、50、100、150、200和300mgL-1的CuNP中,对照组使用罐水,对照组使用浓度为0.3mgL-1的五水硫酸铜(CuSO4.5H2O),一式三份。每10分钟观察一次寄生虫,持续300分钟,并记录死亡率。对于昆虫来说,将寄生虫浸入浓度为50、100、150和300mgL-1的CuNP中。在体外试验结束时,计算每种治疗的驱虫疗效.为了评估鱼类的耐受性和毒性,将它们暴露于浓度为0.6、1.25、2.5、5、10、20和50mgL-1的CuNP中12小时。MIC表明,CuNPs有效抑制嗜水气单胞菌的生长,稀释度为12,500mgL-1,对CuNPs的抑制作用为14.0±1.6mm。驱虫活性的结果表明,两组寄生虫的浓度均具有剂量依赖性,120分钟内最有效浓度为300mgL-1。在毒性测试中,鲤鱼对低浓度表现出耐受性。研究表明CuNP对所研究的病原体有效。然而,它被证明对高浓度的鱼有毒。建议使用低浓度仍需要进一步研究。
This study investigated the in vitro antimicrobial and anthelmintic effect of copper nanoparticles (CuNPs) against the bacterium Aeromonas hydrophila, the monogeneans Dactylogyrus minutus, Dactylogyrus extensus, Gyrodactylus cyprini, and the
cestode Schyzocotyle acheilognathi, as well as their toxicity to Cyprinus carpio Koi. In the antimicrobial in vitro test, the inhibition zone method and minimum inhibitory concentration (MIC) were performed. In order to determine the time and efficacy of monogenean parasite mortality, the parasites were exposed to CuNP concentrations of 20, 50, 100, 150, 200, and 300 mg L-1, and a control group with tank water and one with copper sulphate pentahydrate (CuSO4.5H2O) at a concentration of 0.3 mg L-1, performed in triplicate. The parasites were observed every 10 min for 300 min, and mortality was recorded. For the cestodes, parasites were immersed in CuNP concentrations of 50, 100, 150, and 300 mg L-1. At the end of the in vitro tests, the anthelmintic efficacy of each treatment was calculated. To assess the tolerance and toxicity in fish, they were exposed to CuNP concentrations of 0.6, 1.25, 2.5, 5, 10, 20, and 50 mg L-1 for 12 h. The MIC demonstrated that CuNPs effectively inhibited the growth of A. hydrophila up to a dilution of 12,500 mg L-1 and showed an inhibition zone of 14.0 ± 1.6 mm for CuNPs. The results of anthelmintic activity showed a dose-dependent effect of concentration for both groups of parasites, with the most effective concentration being 300 mg L-1 in 120 min. In the toxicity test, the carps showed tolerance to lower concentrations. The study indicated that CuNPs were effective against the studied pathogens. However, it proved to be toxic to fish at high concentrations. The use of low concentrations is recommended still requires further investigation.