使用标记辅助选择等现代方法对当地兔品种进行遗传改良需要有关具有不同遗传背景的动物的标记性状关联的准确和精确的信息。因此,这项研究旨在估计位于神经肽Y(NPY,g.1778G>C)和磷酸甘油酸突变酶2(PGAM2,c.195C>T)基因在新西兰白人(NZW),Baladi(BR),V线兔子第一个突变是使用高分辨率熔解基因分型的,第二个突变采用PCR-RFLP方法进行基因分型。结果显示,NPY突变与10(V线)和12周龄时的体重之间存在显着关联(NZW,BR,和V线),10至12周龄(BR)的体重增加(BWG),BWG从6到12周龄(NZW,BR,和V线),平均每日收益(新西兰元,BR,和V线,和BR),生长速率(GR)从8到10周(V线),10至12周(BR),和6至12周龄的GR(BR,和V线)。PGAM2突变与体重在10(V线)和12(NZW,和V线)周龄,在所有品种中,在12周龄时都有显著的正累加效应,并与BR中的BWG从8到10和10到12相关联,和6至12周龄的BWG(NZW,和BR),和平均每日收益(新西兰元,和BR),并与8至10周(BR)的GR形式有关,从10到12周(BR,和V线)以及6至12周(BR)。结果强调了这两种突变在生长发育中的重要性,以及将它们视为兔子晚期生长的候选基因的可能性。
Genetic improvement of local rabbit breeds using modern approaches such as marker-assisted selection requires accurate and precise information about marker‒trait associations in animals with different genetic backgrounds. Therefore, this study was designed to estimate the association between two mutations located in the Neuropeptide Y (NPY, g.1778G > C) and Phosphoglycerate Mutase 2 (PGAM2, c.195 C > T) genes in New Zealand White (NZW), Baladi (BR), and V-line rabbits. The first mutation was genotyped using high-resolution melting, and the second mutation was genotyped using the PCR-RFLP method. The results revealed significant associations between the NPY mutation and body weight at 10 (V-line) and 12 weeks of age (NZW, BR, and V-line), body weight gain (BWG) from 10 to 12 weeks of age (BR), BWG from 6 to 12 weeks of age (NZW, BR, and V-line), average daily gain (NZW, BR, and V-line, and BR), growth rate (GR) from 8 to10 weeks (V-line), 10 to 12 weeks (BR), and GR from 6 to 12 weeks of age (BR, and V-line). The PGAM2 mutation was associated with body weight at 10 (V-line) and 12 (NZW, and V-line) weeks of age, with significant positive additive effects at 12 weeks of age in all breeds, and was associated with BWG from 8 to 10 and 10 to 12 in BR, and BWG from 6 to 12 weeks of age (NZW, and BR), and average daily gain (NZW, and BR), and was associated with GR form 8 to 10 weeks (BR), from10 to 12 weeks (BR, and V-line) and from 6 to 12 weeks (BR). The results highlighted the importance of the two mutations in growth development, and the possibility of considering them as candidate genes for late growth in rabbits.