目前的工作重点是从全基因组关联研究的集合中鉴定硬粒小麦QTL热点,对于质量性状,如谷物蛋白质含量和组成,黄色,纤维,晶粒微量元素含量(铁,镁,钾,硒,硫磺,钙,镉),内核玻璃化,粗面粉,和面团质量测试。第一次总共有10个GWAS研究,包括57个品质性状的395个标记-性状关联(MTA),来自9个关联小组的1500多个基因型,用于研究代表广泛的硬粒小麦遗传变异的共有QTL热点。发现MTA分布在所有A和B基因组染色体上,在5B号染色体上观察到的MTA数量最少(15),在7A号染色体上观察到的MTA数量最多45,每个染色体平均28个MTA。MTA在A(48%)和B(52%)基因组上均匀分布,并鉴定了94个QTL热点。QTL的合成图谱也在玉米中进行,Brachypodium,和水稻,和候选基因鉴定允许参与生物过程的基因的关联在质量性状的控制中起主要作用。
The present work focused on the identification of durum wheat QTL hotspots from a collection of genome-wide association studies, for quality traits, such as grain protein content and composition, yellow color, fiber, grain microelement content (iron, magnesium, potassium, selenium, sulfur, calcium, cadmium), kernel vitreousness, semolina, and dough quality test. For the first time a total of 10 GWAS studies, comprising 395 marker-trait associations (MTA) on 57 quality traits, with more than 1,500 genotypes from 9 association panels, were used to investigate
consensus QTL hotspots representative of a wide durum wheat genetic variation. MTA were found distributed on all the A and B genomes chromosomes with minimum number of MTA observed on chromosome 5B (15) and a maximum of 45 on chromosome 7A, with an average of 28 MTA per chromosome. The MTA were equally distributed on A (48%) and B (52%) genomes and allowed the identification of 94 QTL hotspots. Synteny maps for QTL were also performed in Zea mays, Brachypodium, and Oryza sativa, and candidate gene identification allowed the association of genes involved in biological processes playing a major role in the control of quality traits.