■在这项研究中,在长江口潮间带的不同地点调查了中华绒螯蟹(Eriocheirsinensis)幼虫肠道菌群的季节性差异。
■16SrRNA高通量测序技术用于比较和分析来自不同季节的幼蟹肠道中的微生物群落结构。
■结果表明,所有季节和地点的主要微生物门都是变形杆菌,拟杆菌,Firmicutes,和放线菌,占总微生物群的97.1%。成分分析显示,从夏季到冬季,每个站的变形杆菌的相对丰度都在下降,而拟杆菌则表现出相反的趋势。Alpha多样性分析表明,上游地区从夏季到冬季物种丰富度增加(P<0.05),但在下游站点下降(P<0.05),在其他比较中没有观察到显著差异。生物标志物物种分析表明,与秋季和冬季相比,幼蟹在夏季表现出更专业化的微生物群落。共现网络分析显示,与夏季和秋季相比,秋季微生物相互作用网络复杂度较低。功能预测分析表明,微生物群落在氨基酸合成方面仅表现出季节性差异,辅因子,假肢组,电子载体,和维生素的生物合成,芳香族化合物降解,核苷酸和核苷降解,和三羧酸循环途径。
■结果表明,不同部位的微生物群没有显著差异,季节变化是影响中华绒螯蟹肠道菌群差异的主要因素。此外,与秋季和冬季相比,夏季的微生物群落更加复杂。
UNASSIGNED: In this study, the seasonal differences in the intestinal microbiota of Chinese mitten crab (Eriocheir sinensis) larvae were investigated at different sites in the intertidal zone of the Yangtze River Estuary.
UNASSIGNED: 16S rRNA high-throughput sequencing technology was used to compare and analyze the microbial community structure in the intestines of juvenile crab from different seasons.
UNASSIGNED: The results showed that the main microbial phyla in all seasons and sites were Proteobacteria, Bacteroidetes, Firmicutes, and Actinobacteria, which accounted for 97.1% of the total microbiota. Composition analysis revealed that the relative abundance of Proteobacteria decreased from summer to winter at each station, whereas Bacteroidetes showed the opposite trend. Alpha diversity analysis showed that species richness increased from summer to winter at the upstream site (P < 0.05), but decreased at the downstream site (P < 0.05), with no significant differences observed in other comparisons. Biomarker species analysis showed that juvenile crab exhibited a more specialized microbial community in summer compared with autumn and winter. Co-occurrence network analysis revealed that microbial interaction network complexity was lower in autumn compared with summer and autumn. Functional prediction analysis showed that the microbial community only exhibited seasonal differences in amino acid biosynthesis, cofactor, prosthetic group, electron carrier, and vitamin biosynthesis, aromatic compound degradation, nucleotide and nucleoside degradation, and tricarboxylic acid cycle pathways.
UNASSIGNED: The results indicated that the microbiota did not significantly differ among sites, and seasonal variation was a main factor influencing the differences in intestinal microbiota of Chinese mitten juvenile crab. Moreover, the microbial community was more complex in summer compared with autumn and winter.