PDF(Pubmed)
Abstract：
This study used berberine hydrochloride to treat the Asian paddle crab, Charybdis japonica infected with the Gram-negative bacterium Aeromonas hydrophila at concentrations of 0, 100, 200 and 300 mg/L. The effect of berberine hydrochloride on the survival rate and gut microbiota of C. japonica was investigated. Berberine hydrochloride improved the stability of the intestinal flora, with an increase in the abundance of probiotic species and a decrease in the abundance of both pathogenic bacteria after treatment with high concentrations of berberine hydrochloride. Berberine hydrochloride altered peroxidase activity (POD), malondialdehyde (MDA), and lipid peroxidation (LPO) in the intestinal tract compared to the control. Berberine hydrochloride could modulate the energy released from the enzyme activities of hexokinase (HK), phosphofructokinase (PFK), and pyruvate kinase (PK) in the intestinal tract of C. japonica infected with A. hydrophila. Zona occludens 1 (ZO-1), Zinc finger E-box binding homeobox 1 (ZEB1), occludin and signal transducer, and activator of transcription5b (STAT5b) expression were also increased, which improved intestinal barrier function. The results of this study provide new insights into the role of berberine hydrochloride in intestinal immune mechanisms and oxidative stress in crustaceans.
摘要：
本研究使用盐酸小檗碱治疗亚洲桨蟹，以0、100、200和300mg/L的浓度感染革兰氏阴性细菌嗜水气单胞菌研究了盐酸小檗碱对日本花青素成活率和肠道菌群的影响。盐酸小檗碱提高了肠道菌群的稳定性，用高浓度盐酸小檗碱处理后，益生菌的丰度增加，两种致病菌的丰度降低。盐酸小檗碱改变过氧化物酶活性（POD），丙二醛（MDA），与对照相比，肠道中的脂质过氧化（LPO）。盐酸小檗碱可以调节己糖激酶（HK）酶活性释放的能量，磷酸果糖激酶（PFK），和丙酮酸激酶（PK）在感染嗜水气单胞菌的日本血吸虫的肠道。Zona闭塞1（ZO-1），锌指E盒结合homeobox1(ZEB1),occludin和信号换能器，转录激活因子5b(STAT5b)表达也增加，改善肠屏障功能。本研究结果为盐酸小檗碱在甲壳类动物肠道免疫机制和氧化应激中的作用提供了新的见解。
