UNASSIGNED: Microplastics (MPs) are pervasive pollutants in the marine environment, exhibiting persistence in coastal sediment over extended periods. However, the mechanism of their uptake by marine organisms and distribution in habitat is less understood. The objective of the present study was to investigate the presence of MP contamination in burrow sediment, feeding pellets, and tissue of Dotilla blanfordi in the Gulf of Kachchh, Gujarat State.
UNASSIGNED: A total of 500 g of burrow sediment, 100 g of feeding pellets, and body tissue of 10 resident D. blanfordi were pooled as one replica. Such seven replicas from each site were analyzed for MP extraction from three sites, including Asharmata, Mandvi, and Serena, located in the Gulf of Kachchh. The standard protocol was used during the analysis of the collected samples in order to isolate MPs.
UNASSIGNED: The abundance of MP was found higher in burrow sediment, feeding pellets and tissue of D. blanfordi at study site Mandvi, followed by Serena and Asharmata. The abundance of MP was found higher in D. blanfordi tissue, followed by burrow sediment and feeding pellet. A significant variation was observed in MP abundance among burrow sediment, feeding pellets, and tissue. MPs with various shapes (fiber, film, and fragment), sizes (1-2, 2-3, 3-4, and 4-5 mm), and colors (blue, green, black, pink, purple, red transparent) were recorded from all the study sites. Polyurethane and polyvinyl chloride were recognized as the chemical profile of the extracted MPs. The current investigation revealed greater accumulation of MPs in D. blanfordi\'s tissues compared to sediment and pellets, suggesting a risk of MP contamination in marine benthic fauna with a greater rate of bioaccumulation. D. blanfordi plays a significant role as a structuring agent for MP distribution in the intertidal flat through burrowing activity.

Carotenoid cleavage oxygenases can cleave carotenoids into a range of biologically important products. Carotenoid isomerooxygenase (NinaB) and β, β-carotene 15, 15\'-monooxygenase (BCO1) are two important oxygenases. In order to understand the roles that both oxygenases exert in crustaceans, we first investigated NinaB-like (EsNinaBl) and BCO1-like (EsBCO1l) within the genome of Chinese mitten crab (Eriocheir sinensis). Their functions were then deciphered through an analysis of their expression patterns, an in vitro β-carotene degradation assay, and RNA interference. The results showed that both EsNinaBl and EsBCO1l contain an RPE65 domain and exhibit high levels of expression in the hepatopancreas. During the molting stage, EsNinaBl exhibited significant upregulation in stage C, whereas EsBCO1l showed significantly higher expression levels at stage AB. Moreover, dietary supplementation with β-carotene resulted in a notable increase in the expression of EsNinaBl and EsBCO1l in the hepatopancreas. Further functional assays showed that the EsNinaBl expressed in E. coli underwent significant changes in its color, from orange to light; in addition, its β-carotene cleavage was higher than that of EsBCO1l. After the knockdown of EsNinaBl or EsBCO1l in juvenile E. sinensis, the expression levels of both genes were significantly decreased in the hepatopancreas, accompanied by a notable increase in the redness (a*) values. Furthermore, a significant increase in the β-carotene content was observed in the hepatopancreas when EsNinaBl-mRNA was suppressed, which suggests that EsNinaBl plays an important role in carotenoid cleavage, specifically β-carotene. In conclusion, our findings suggest that EsNinaBl and EsBCO1l may exhibit functional co-expression and play a crucial role in carotenoid cleavage in crabs.

Modified clay compounds are used globally as a method of controlling harmful algal blooms, and their use is currently under consideration to control Karenia brevis blooms in Florida, USA. In 1400 L mesocosm tanks, chemical dynamics and lethal and sublethal impacts of MC II, a polyaluminum chloride (PAC)-modified kaolinite clay, were evaluated over 72 h on a benthic community representative of Sarasota Bay, which included blue crab (Callinectes sapidus), sea urchin (Lytechinus variegatus), and hard clam (Mercenaria campechiensis). In this experiment, MC II was dosed at 0.2 g L-1 to treat bloom-level densities of K. brevis at 1 × 106 cells L-1. Cell removal in MC II-treated tanks was 57% after 8 h and 95% after 48 h. In the water column, brevetoxin analogs BTx-1 and BTx-2 were found to be significantly higher in untreated tanks at 24 and 48 h, while in MC II-treated tanks, BTx-3 was found to be higher at 48 h and BTx-B5 was found to be higher at 24 and 48 h. In MC II floc, we found no significant differences in BTx-1 or BTx-2 between treatments for any time point, while BTx-3 was found to be significantly higher in the MC II-treated tanks at 48 and 72 h, and BTx-B5 was higher in MC II-treated tanks at 24 and 72 h. Among various chemical dynamics observed, it was notable that dissolved phosphorus was consistently significantly lower in MC II tanks after 2 h, and that turbidity in MC II tanks returned to control levels 48 h after treatment. Dissolved inorganic carbon and total seawater alkalinity were significantly reduced in MC II tanks, and partial pressure of CO2 (pCO2) was significantly higher in the MC II-only treatment after 2 h. In MC II floc, particulate phosphorus was found to be significantly higher in MC II tanks after 24 h. In animals, lethal and sublethal responses to MC II-treated K. brevis did not differ from untreated K. brevis for either of our three species at any time point, suggesting MC II treatment at this dosage has negligible impacts to these species within 72 h of exposure. These results appear promising in terms of the environmental safety of MC II as a potential bloom control option, and we recommend scaling up MC II experiments to field trials in order to gain deeper understanding of MC II performance and dynamics in natural waters.

Complete mitochondrial genomes (mitogenomes) can provide important information regarding the molecular evolution and phylogenetic relationships of marine invertebrates, especially in Brachyura. Only one Cancroidea species of mitogenomes has been sequenced before; in this research, the mitogenomic characteristics of Metacarcinus magister (Cancridae: Cancroidea) are newly studied. The length of the M. magister mitogenome was 48,820 bp, and it contained the typical 13 protein-coding genes, 2 ribosomal RNA genes, and 22 transfer RNA genes. We performed a series of analyses on the characteristics of the mNCR of M. magister. The phylogenetics, life circumstances, and selective pressures were all analyzed to explain the formation of this length, which revealed the length of the M. magister mitogenome to be approximately three times greater than the normal length of Brachyuran mitogenomes. Phylogenetic analyses based on a dataset of 215 Decapodan mitogenomes indicated that all Eriphioidea crabs were clustered together as a group. Moreover, the rearrangement mechanism of the Cancroidea species was predicted to provide stronger evidence for the phylogenetic analysis. In general, the results obtained in this study will contribute to a better understanding of the cause of the unusual length of the M. magister mitogenome and provide new insights into the phylogeny of Brachyura.

Crustaceans possess a range of sensory organs crucial in sensory perception, communication, and various ecological functions. Understanding morphological and functional differences in antennae among species could validate taxonomic differentiation and ecological adaptations. The antennae morphology and ultrastructure of mud crab species within the Scylla genus are poorly understood, and their role in ecological adaptation and species differentiation remains unexplored. This study aimed to describe and compare the morphology and ultrastructure of antennae in Scylla olivacea, Scylla tranquebarica, and Scylla paramamosain. Antennae were carefully excised from each crab and subjected to morphological, morphometric, and ultrastructural analysis. The study revealed that the antennae of Scylla species exhibit similar overall morphology, with a series of segments that tapered toward the upper end. All species possess non-branched single setae on the upper end of each segment. The number of antennae segments varied between species, with S. paramamosain having significantly more segments than S. olivacea. Additionally, the length and width of antenna segments differed among the species, with S. tranquebarica having a rougher antenna surface compared to S. olivacea and S. paramamosain. Our findings suggest that Scylla\'s antennae are distinct between species, especially in the number of segments and setae size. Such difference might be related to ecological adaptation. The role of antennae in sensory perception and social behavioral cues in mud crabs warrants further investigation. This study serves as a foundational reference for future research on the taxonomy, ecological adaptation, and sensory behaviors in the Scylla genus. RESEARCH HIGHLIGHTS: Variations and similarities in morphology and ultrastructure of three Scylla species can be found in the antennae. Scylla paramamosain had significantly higher number of segments than Scylla olivacea in morphology feature. The antennae surface of Scylla tranquebarica was rougher than that of S. olivacea and S. paramamosain. Antennae of three Scylla species possess non-branched single setae.

The intensification of production practices in the aquaculture industry has led to the indiscriminate use of antibiotics to combat diseases and reduce costs, which has resulted in environmental pollution, posing serious threats to aquaculture sustainability and food safety. However, the toxic effect of florfenicol (FF) exposure on the hepatopancreas of crustaceans remains unclear. Herein, by employing Chinese mitten crab (Eriocheir sinensis) as subjects to investigate the toxic effects on histopathology, oxidative stress, apoptosis and microbiota of hepatopancreas under environment-relevant (0.5 and 5 μg/L), and extreme concentrations (50 μg/L) of FF. Our results revealed that the damage of hepatopancreas tissue structure caused by FF exposure in a dose-and time-dependent manner. Combined with the increased expression of apoptosis-related genes (Caspase 3, Caspase 8, p53, Bax and Bcl-2) at mRNA and protein levels, activation of catalase (CAT) and superoxide dismutase (SOD), and malondialdehyde (MDA) accumulation, FF exposure also induced oxidative stress, and apoptosis in hepatopancreas. Interestingly, 7 days exposure triggered more pronounced toxic effect in crabs than 14 days under environment-relevant FF concentration. Integrated biomarker response version 2 (IBRv2) index indicated that 14 days FF exposure under extreme concentration has serious toxicity effect on crabs. Furthermore, 14 days exposure to FF changed the diversity and composition of hepatopancreas microbiota leading remarkable increase of pathogenic microorganism Spirochaetes following exposure to 50 μg/L of FF. Taken together, our study explained potential mechanism of FF toxicity on hepatopancreas of crustaceans, and provided a reference for the concentration of FF to be used in culture of Chinese mitten crab.

Scylla serrata plays a crucial role in India\'s seafood exports yet there exists limited understanding on the occurrence of microplastics (MPs) in these crabs. In this baseline study, we examined the presence of microplastics in the digestive tracts of S. serrata collected from the Kota mangroves, southwestern coast of India. Our analysis revealed the presence of 264 MPs in all the samples with an average (± standard deviation) of 29.33 (±11.53) MPs/Individual. The most dominant categories were fibres (98.86 %) and fragments (1.14 %). Primarily 0.1-0.3 mm (50.90 %) and 0.3-1 mm (37.65 %) size range dominated. The predominant polymers were polypropylene (33.71 %), high-density polyethylene (31.44 %), and polyethylene terephthalate (17.80 %). Scanning electron microscopy revealed extensive weathering on the surface of the microplastics. Risk assessments indicated severe risks to S. serrata due to microplastic ingestion emphasizing the need to protect delicate ecosystems like mangroves and the biota within.

Early pioneering studies by Autrum on terrestrial arthropods first revealed that the visual systems of arthropods reflected their lifestyles and habitats. Subsequent studies have examined and confirmed Autrum\'s hypothesis that visual adaptions are driven by predator-prey interactions and activity cycles, with rapidly moving predatory diurnal species generally possessing better temporal resolution than slower moving nocturnal species. However, few studies have compared the vision between diurnal herbivores and nocturnal predators. In this study, the visual physiology of a nocturnal fast-moving predatory crab, the Atlantic ghost crab (Ocypode quadrata) and a diurnal herbivorous crab, the mangrove tree crab (Aratus pisonii), was examined. Spectral sensitivity, irradiance sensitivity and temporal resolution of the crabs were quantified using the electroretinogram (ERG), while the spatial resolution was calculated utilizing morphological methods. Both O. quadrata and A. pisonii had a single dark-adapted spectral sensitivity peak (494 and 499 nm, respectively) and chromatic adaptation had no effect on their spectral sensitivity, indicating that both species have monochromatic visual systems. The temporal resolution of O. quadrata was not significantly different from that of A. pisonii, but O. quadrata did possess a significantly greater spatial resolution and irradiance sensitivity. Both species possess an acute zone in the anterior region of their eyes. The data presented in this study will aid in the current understanding of the correlation between visual physiology and the life history of the animal.

Mesanophrys sp. is a parasitic ciliate that invades and destroys the hemocytes of the swimming crab (Portunus trituberculatus). In the present study, we employed an in vitro model to elucidate how Mesanophrys sp. destroys crab hemocytes. We also evaluated the relationship between the parasite\'s density, the destruction rate of the hemocytes, and the rapid proliferation pattern of parasites in host crabs. We found that the survival rate and cell integrity of crab hemocytes decreased with an increase in Mesanophrys sp. density, depicting a negative correlation between hemocyte viability and parasite density. Further analyses revealed that crab hemocytes could resist destruction by a low density (10 ind/mL) of Mesanophrys sp. for a long time (60 h). Mesanophrys sp. and its culture medium (containing the ciliate secretions) destroy the host hemocytes. The natural population growth rate of Mesanophrys sp. decreased with an increase in the parasite density, but the Mesanophrys sp. density did not affect the generation time of the parasites. In summary, Mesanophrys sp. can destroy crab hemocytes, and the degree of destruction is directly proportional to the parasite density. The resistance of crab hemocytes to Mesanophrys sp. decreased gradually with an increase in the parasite density.

High microplastic concentrations in the Delaware Bay have prompted concern regarding harm to local species. We consider the extent to which the zooplankton is exposed to bay-derived microplastics, focusing on Atlantic blue crabs (Callinectes sapidus) during offshore larval migration. We simulate regional flow fields for a spawning season in the Delaware coastal system to advect passive Lagrangian microplastic and zooplankton tracers. Microplastic exposure levels are estimated from tracer distributions. Field sampling of zooplankton and microplastic concentrations for the Delaware Bay mouth and the adjacent shelf in August 2020 is utilized to appraise model performance. Three mechanisms elevating microplastics exposure are identified: zooplankton transport into microplastic-laden tidelines, displacement of microplastics into the buoyant outflow current, and aggregation in offshore plume fronts. Organization via the above mechanisms substantially enhance microplastic exposures over zooplankton migrations (by an average factor of at least 3.8).