骨缺损的临床治疗包括同种异体骨移植和自体骨移植。然而,它们都有自己的局限性,且适用范围有限。近年来,基于多种材料的骨组织工程支架得到了很好的发展,并取得了良好的骨再生能力。然而,大多数支架材料总是面临着生物毒性高、导致炎症和生物活性差,限制了骨再生效果,延长了骨再生时间。在我们的工作中,我们制备了羟基磷灰石,促红细胞生成素(EPO),和成骨生长肽(OGP)通过静电纺丝共掺杂I型胶原蛋白(ColI)多肽纳米纤维膜(NFM)。在细胞实验中,复合NFMs具有较低的细胞毒性,促进大鼠骨髓间充质干细胞的成骨分化。定量实时聚合酶链反应和碱性磷酸酶染色证实了成骨基因的高表达,和茜素红S染色直接证实了钙结节的出现。在动物实验中,负载的羟基磷灰石在缺陷中心形成了多个独立的矿化中心。在一级上校的推动下,EPO,OGP,骨骼沿着矿化中心以及向内的缺损边缘继续生长,骨缺损在大约两个月内完全再生。血液学和组织学分析证明了实验的安全性。这种设计通过模拟骨成分来促进骨再生,引入矿化中心和信号分子,可以缩短维修时间,提高修复效果,并在未来具有良好的实践前景。
The clinical treatment of bone defects includes allogeneic bone transplantation and autologous bone transplantation. However, they all have their own limitations, and the scope of application is limited. In recent years, bone tissue engineering scaffolds based on a variety of materials have been well developed and achieved good bone regeneration ability. However, most scaffold materials always face problems such as high biotoxicity, leading to inflammation and poor bioactivity, which limits the bone regeneration effect and prolongs the bone regeneration time. In our work, we prepared hydroxyapatite, erythropoietin (EPO), and osteogenic growth peptide (OGP) codoped type-I collagen (Col I) polypeptide nanofiber membranes (NFMs) by electrostatic spinning. In cell experiments, the composite NFMs had low cytotoxicity and promoted osteogenic differentiation of rat bone marrow mesenchymal stem cells. Quantitative real-time polymerase chain reaction and alkaline phosphatase staining confirmed the high expression of osteogenic genes, and alizarin red S staining directly confirmed the appearance of calcium nodules. In animal experiments, the loaded hydroxyapatite formed multiple independent mineralization centers in the defect center. Under the promotion of Col I, EPO, and OGP, the bone continued to grow along the mineralization centers as well as inward the defect edge, and the bone defect completely regenerated in about two months. The hematological and histological analyses proved the safety of the experiments. This kind of design to promote bone regeneration by simulating bone composition, introducing mineralization center and signal molecules, can shorten repair time, improve repair effect, and has good practical prospects in the future.