PDF(Pubmed)
Abstract：
It\'s imperative to create a more ideal biological scaffold for bone defect repair. Calcium phosphate bone cements (CPC) could be used as a scaffold. Some ingredients and osteogenic factors could be added to improve its poor mechanical properties and biological activity. As a macromolecule extracted from traditional Chinese medicine, Hedysarum polysaccharides (HPS) would significantly promote the osteogenic activity of bone biomaterials. Zirconium oxide and starch were added to the solid phase and citric acid was added to the liquid phase to optimize CPC. HPS was loaded onto the scaffold as an osteogenic factor, and the prepared CPS + HPS was characterized. Further, the cytocompatibility of CPS + HPS was assessed according to activity, differentiation, and calcification in neonatal rat calvarial osteoblasts, and the biosafety of CPS + HPS was evaluated according to acute toxicity, pyrogen, sensitization, and hemolysis. The success of CPS + HPS in repairing bone defects was evaluated by using a rabbit femur implantation experiment. After optimization, CPS-20-CA-5 containing 10% starch and 5% citric acid displayed the highest mechanical strength of 28.96 ± 0.03 MPa. HPS-50 was demonstrated to exert the best osteogenic effect. The combination of CPS + HPS achieved HPS-loaded CPC. Material characterization, cytocompatibility, biosafety, and femoral implantation experiments indicated that CPS + HPS possessed better pressure resistance and improved osteogenic ability in bone defect repair.CPS + HPS demonstrated effective pressure resistance and superior osteogenic ability, which may be of great significance for bone defects and bone tissue engineering to promote bone regeneration and repair.
摘要：
为骨缺损修复创造更理想的生物支架势在必行。磷酸钙骨水泥(CPC)可用作支架。可以添加一些成分和成骨因子来改善其不良的机械性能和生物活性。作为一种从中药中提取的大分子,Hedysarum多糖（HPS）可以显着促进骨生物材料的成骨活性。将氧化锆和淀粉添加到固相中,并将柠檬酸添加到液相中以优化CPC。将HPS作为成骨因子加载到支架上，并对所制备的CPS+HPS进行了表征。Further,根据活性评估CPS+HPS的细胞相容性，分化,新生大鼠颅骨成骨细胞钙化，并根据急性毒性评估CPS+HPS的生物安全性，热原，致敏,和溶血。通过使用兔股骨植入实验评估CPSHPS修复骨缺损的成功。优化后,含有10%淀粉和5%柠檬酸的CPS-20-CA-5显示出28.96±0.03MPa的最高机械强度。HPS-50被证明发挥最佳的成骨作用。CPS+HPS的组合实现了HPS负载的CPC。材料表征,细胞相容性,生物安全,股骨植入实验表明，CPSHPS具有更好的抗压能力，可改善骨缺损修复的成骨能力。CPS+HPS表现出有效的耐压性和优越的成骨能力，骨缺损和骨组织工程促进骨再生修复具有重要意义。
