曲霉属由大量医学和环境相关的物种组成。按系列分类的曲霉属在环境中无处不在,包括机会性病原体sydowii曲霉,与甲癣和浅表皮肤感染有关。尽管经常有关于sydowii和相关系列Versicolores物种的临床报道,抗真菌药敏数据很少,妨碍最佳治疗选择和随后的患者结局。这里,我们采用了基于微肉汤稀释的抗真菌药敏试验(AFST)对一组155系列Versicoles菌株使用常见的抗真菌药两性霉素B,伊曲康唑,伏立康唑,泊沙康唑,伊沙武康唑和米卡芬净加入卢立康唑和奥洛芬。所有菌株均使用部分钙调蛋白基因测序进行鉴定,145个是A.sydowii,七个a.creber和三个a.versicolor,使用最新的分类学见解。总的来说,测试的抗真菌药对整个菌株集合都有效。与烟曲霉相比,一些菌株的唑类和两性霉素B的MIC略有升高。服用卢立康唑和奥洛芬后,这里第一次报道,显示出最高的体外活性,使这些抗真菌药物成为有趣的替代药物,但临床研究有必要用于未来的治疗用途。
The genus
Aspergillus consists of a vast number of medically and environmentally relevant species.
Aspergillus species classified in series Versicolores are ubiquitous in the environment and include the opportunistic pathogen
Aspergillus sydowii, which is associated with onychomycosis and superficial skin infections. Despite frequent clinical reports of A. sydowii and related series Versicolores species, antifungal susceptibility data are scarce, hampering optimal treatment choices and subsequent patient outcomes. Here, we employed antifungal susceptibility testing (AFST) based on microbroth dilution on a set of 155 series Versicolores strains using the common antifungals amphotericin B, itraconazole, voriconazole, posaconazole, isavuconazole and micafungin with the addition of luliconazole and olorofim. All strains were identified using partial calmodulin gene sequencing, with 145 being A. sydowii, seven A. creber and three A. versicolor, using the latest taxonomic insights. Overall, tested antifungals were potent against the entire strain collection. In comparison to A. fumigatus, azole and amphotericin B MICs were slightly elevated for some strains. AFST with luliconazole and olorofim, here reported for the first time, displayed the highest in vitro activity, making these antifungals interesting alternative drugs but clinical studies are warranted for future therapeutic use.