PDF(Pubmed)
Abstract：
This study investigated the potential of endophytic fungi to produce paclitaxel (Taxol®), a potent anticancer compound widely employed in chemotherapy. This research aimed to identify, confirm, and characterize endophytic fungi capable of paclitaxel (PTX) production and assess their paclitaxel yield. Additionally, it aimed to investigate factors influencing paclitaxel production. A total of 100 endophytic fungal isolates were collected and identified from the roots of Artemisia judaica. Aspergillus fumigatiaffinis exhibited the highest PTX production (26.373 μg L-1) among the isolated endophytic fungi. The strain was identified as A. fumigatiaffinis (Accession No. PP235788.1). Molecular identification confirmed its novelty, representing the first report of PTX production by A. fumigatiaffinis, an endophyte of Artemisia judaica. Optimization through full factorial design of experiments (DOE) and response surface methodology (RSM) significantly enhanced PTX production to 110.23 μg L-1 from 1 g of dry weight of the fungal culture under optimal conditions of pH 8.0, 150 μg L-1 becozyme supplementation, and 18 days of fermentation in potato dextrose broth. The presence of paclitaxel was confirmed using thin layer chromatography, high performance liquid chromatography, and gas chromatography-mass spectrometry. These findings maximize the role of endophytic fungus to produce a secondary metabolite that might be able to replace the chemically produced PTX and gives an opportunity to provide a sustainable source of PTX eco-friendly at high concentrations. KEY POINTS: • Endophytic fungi, like A. fumigatiaffinis, show promise for eco-friendly paclitaxel production • Optimization strategies boost paclitaxel yield significantly, reaching 110.23 μg L -1 • Molecular identification confirms novelty, offering a sustainable PTX source.
摘要：
这项研究调查了内生真菌产生紫杉醇（Taxol®）的潜力，广泛用于化疗的一种有效的抗癌化合物。这项研究旨在确定，确认,并表征能够产生紫杉醇（PTX）的内生真菌并评估其紫杉醇产量。此外,探讨紫杉醇生产的影响因素。从红蒿根中收集并鉴定了100株内生真菌。在分离的内生真菌中，烟曲霉的PTX产量最高（26.373μgL-1）。该菌株被鉴定为A.fumigatiaffinis（登录号。PP235788.1)。分子鉴定证实了它的新颖性，代表A.fumigatiaffinis的PTX生产的第一份报告，紫蒿的一种内生菌。通过全因子实验设计（DOE）和响应面法（RSM）进行优化，在pH8.0，150μgL-1的最佳条件下，从1g真菌培养物的干重将PTX产量显着提高到110.23μgL-1补充酶，在马铃薯葡萄糖肉汤中发酵18天。使用薄层色谱法确认紫杉醇的存在，高效液相色谱法,和气相色谱-质谱法。这些发现最大限度地发挥了内生真菌产生次级代谢产物的作用，该代谢产物可能能够替代化学产生的PTX，并提供了在高浓度下提供可持续的PTX生态友好来源的机会。关键词：•内生真菌，比如熏蒸，显示出环保紫杉醇生产的希望•优化策略显着提高紫杉醇产量，达到110.23μgL-1•分子鉴定证实了新颖性，提供可持续的PTX来源。
