■Anisakis是全球分布的,海洋寄生线虫可能导致人类健康问题,包括呕吐等症状,急性腹泻,和过敏反应。作为寄生线虫,主要影响患者的消化道,肠道蠕虫可以通过身体接触直接与宿主微生物群相互作用,化学品,或者营养竞争。人们普遍认为宿主微生物群在免疫调节中起着至关重要的作用。
■通过分子生物学鉴定了从海鱼腹腔收集的线虫,并在大鼠中人工感染了活虫。通过基于大鼠血清和蠕虫提取物的间接ELISA确定感染。收集粪便用于基于16SrDNA的微生物群多样性分析。
■基于ITS序列的分子生物学鉴定将收集的线虫鉴定为A.pegreffii。通过基于人工感染大鼠的血清和蠕虫提取的间接ELISA来确定人工感染的成功。微生物多样性分析表明,总共产生了773只ASV,和PCoA显示感染组与对照组有区别。对照组包含五个特征属(PrevotellaceaeNK3B31组,Turicibacter,严格的感觉梭菌1,Stoquefichus念珠菌,Lachnospira)和感染组包含9个特征属(啮齿细菌,Christensenella,Dubosiella,链球菌,厌氧等离子体,乳球菌,乳头状杆菌,Desulfovibrio,罗斯布里亚)。根据Wilcoxon检验,发现四个过程是重要的:细菌分泌系统,细菌入侵上皮细胞,细菌趋化性,和ABC运输机。
■这项研究首次分析了感染A.pegreffii的大鼠肠道微生物群的多样性,并确定了由大鼠肠道中的感染引起的代谢和免疫的损害和调节。研究结果为进一步研究宿主-蠕虫-微生物的相关性奠定了基础。
UNASSIGNED: Anisakis are globally distributed, marine parasitic nematodes that can cause human health problems, including symptoms such as vomiting, acute diarrhea, and allergic reactions. As parasitic nematodes that primarily affect the patient\'s digestive tract, intestinal helminths can interact directly with the host microbiota through physical contact, chemicals, or nutrient competition. It is widely accepted that the host microbiota plays a crucial role in the regulation of immunity.
UNASSIGNED: Nematodes collected from the abdominal cavity of marine fish were identified by molecular biology and live worms were artificially infected in rats. Infection was determined by indirect ELISA based on rat serum and worm extraction. Feces were collected for 16S rDNA-based analysis of microbiota diversity.
UNASSIGNED: Molecular biology identification based on ITS sequences identified the collected nematodes as A. pegreffii. The success of the artificial infection was determined by indirect ELISA based on serum and worm extraction from artificially infected rats. Microbiota diversity analysis showed that a total of 773 ASVs were generated, and PCoA showed that the infected group was differentiated from the control group. The control group contained five characterized genera (Prevotellaceae NK3B31 group, Turicibacter, Clostridium sensu stricto 1, Candidatus Stoquefichus, Lachnospira) and the infected group contained nine characterized genera (Rodentibacter, Christensenella, Dubosiella, Streptococcus, Anaeroplasma, Lactococcus, Papillibacter, Desulfovibrio, Roseburia). Based on the Wilcoxon test, four processes were found to be significant: bacterial secretion system, bacterial invasion of epithelial cells, bacterial chemotaxis, and ABC transporters.
UNASSIGNED: This study is the first to analyze the diversity of the intestinal microbiota of rats infected with A. pegreffii and to determine the damage and regulation of metabolism and immunity caused by the infection in the rat gut. The findings provide a basis for further research on host-helminth-microbe correlationships.