Abstract：
The study aimed to perform the molecular identification of Anisakis larvae in commercial fish from the coast of the Canary Islands and to provide data on their infection level for the host and the species of this nematode parasite that we could find in several species of commercial interest in the Canary Archipelago. Fish specimens (n = 172) from the Canary coasts were examined for parasites. In total, 495 larvae were identified; PCR was carried out for the entire ITS rDNA and cox2 mtDNA region, obtaining sixteen sequences for the entire ITS rDNA region and fifteen for the cox2 mtDNA, this being the first contribution of nucleotide sequences of Anisakis species of fish caught from the Canary Islands. An overall prevalence of 25% was obtained in the fish analyzed, and five species of Anisakis were identified, these being Anisakis simplex (s.s.), Anisakis pegreffii, Anisakis physeteris, Anisakis nascettii and Anisakis typica and the hybrid Anisakis simplex x Anisakis pegreffii. The results obtained in this study have relevance for public health, since the pathology will depend on the species of Anisakis, so it is important to know the health status of fish in the waters of the Canary Islands to assure a safer consumption and take adequate measures, in addition to the provision of epidemiological data.
摘要：
该研究旨在对加那利群岛海岸的商业鱼类中的Anisakis幼虫进行分子鉴定，并提供有关其宿主和该线虫寄生虫物种的感染水平的数据，我们可以在几种具有商业价值的物种中找到。加那利群岛。对来自金丝雀海岸的鱼类标本（n=172）进行了寄生虫检查。总的来说,鉴定了495只幼虫；对整个ITSrDNA和cox2mtDNA区域进行了PCR，获得整个ITSrDNA区域的16个序列和cox2mtDNA的15个序列，这是从加那利群岛捕获的Anisakis鱼类的核苷酸序列的第一个贡献。在分析的鱼类中获得了25%的总体患病率，并鉴定了五种异尖异语，这些是Anisakis单形(s.s.)，异语女神pegreffii，植物异语，异isakisnascettii和典型的异isakis和杂种异isakis单形x异isakispegreffii。这项研究获得的结果与公共卫生有关，因为病理学将取决于异尖病菌的种类,因此，重要的是要了解加那利群岛水域鱼类的健康状况，以确保更安全的消费并采取适当的措施，除了提供流行病学数据。
