Abstract：
Effects of temperature on development of eggs, recently hatched larvae and L3 larvae of the marine parasitic nematodes Anisakis simplex sensu stricto (s.s.) and A. pegreffii were examined in vitro. The eggs of A. simplex s.s. hatched at 3-25 °C and those of A. pegreffii hatched at 3-27 °C. Days before hatching varied between 2 days at 25 °C and 35-36 days at 3 °C in A. simplex s.s. and between 2 and 3 days at 27 °C and 65 days at 3 °C in A. pegreffii. Hatching rates of A. simplex s.s. were maintained high at temperatures between 3 and 25 °C but decreased to 0% at 27 °C. In contrast, those of A. pegreffii were lowest particularly at 3 °C, but also at 27 °C. The mean 50% survivals of hatched larvae ranged from 5.3 days at 25 °C to 82.3 days at 9 °C in A. simplex s.s., while in A. pegreffii it ranged from 1.2 days at 27 °C to 77.2 days at 9 °C. L3 larvae of A. pegreffii exhibited higher survival rates and activity than those of A. simplex s.s., particularly at 20 and 25 °C. These results suggest that the early stages of A. simplex s.s. are more adapted to lower temperatures whereas those of A. pegreffii are more tolerant to warm environments, which may correspond to their distribution patterns in Japan and Europe.
摘要：
温度对鸡蛋发育的影响,最近在体外检查了海洋寄生线虫Anisakissimplexsensustricto（s.s.）和A.pegreffii的孵化幼虫和L3幼虫。A.simplexs.s.的卵在3-25°C孵化，A.pegreffii的卵在3-27°C孵化。孵化前几天在A.simplexs.s.中在25°C下2天和在3°C下35-36天之间变化。在A.pegrefii中在27°C下2至3天和在3°C下65天之间变化。A.simplexs.s.的孵化率在3至25°C的温度下保持较高，但在27°C时降至0％。相比之下,A.pegreffii的温度最低，特别是在3°C时，但也在27°C。在A.simplexs.s.中，孵化幼虫的平均50％存活率从25°C的5.3天到9°C的82.3天不等。而在A.pegreffii中，它的范围从27°C的1.2天到9°C的77.2天。A.pegreffii的L3幼虫表现出比A.simplexs.s.更高的存活率和活性，特别是在20和25℃。这些结果表明，A.simplexs.s.的早期阶段更适应较低的温度，而A.pegreffii的早期阶段更耐受温暖的环境，这可能与它们在日本和欧洲的分布模式相对应。
