背景:激素受体通过与配体结合发挥其功能,这导致由基因组或非基因组机制介导的细胞信号传导激活。tick虫及其宿主Bostaurus的内在分子通讯包括涉及激素的内分泌调节。在本研究中,我们对R.microplus膜相关孕酮受体(RmMAPRC)进行了分子和计算机模拟分析。
方法:使用生物信息学工具分析RmMAPRC蛋白序列,通过三维建模和分子对接对其结构进行表征。半定量逆转录和聚合酶链反应(sqRT-PCR)评估了蜱器官和胚胎细胞中RmMAPRC基因的存在和相对表达。
结果:RmMAPRC在唾液腺中的相对表达,卵巢,胚胎细胞显示3%的过表达,13%,24%,分别。生物信息学分析表明,RmMAPRC对应于〜23.7kDa的孕酮受体膜成分1(RmPGRMC1),具有N末端跨膜结构域和C末端细胞色素b5样血红素/类固醇结合结构域。对接结果表明RmPGRMC1可以与孕酮(P4)结合,一些孕激素,和P4拮抗剂。系统发育重建表明,Rhipicephalusspp。MAPRC受体聚集在包括阑尾R.在内的进化枝中,R.sanguineus,和R.microplus(RmMAPRC),哺乳动物和蠕虫MAPRC受体聚集在远离蜱的两个独立的分支中。
结论:RmPGRMC1的存在突出了作为节肢动物寄生虫成功的保守适应性机制的调控的重要性,使其成为滴答控制的目标。
BACKGROUND: Hormone receptors exert their function through binding with their ligands, which results in cellular signaling activation mediated by genomic or non-genomic mechanisms. The intrinsic molecular communication of tick Rhipicephalus microplus and its host Bos taurus comprises an endocrine regulation involving hormones. In the present study, we performed a molecular and in silico analysis of a Membrane Associated Progesterone Receptor in R. microplus (RmMAPRC).
METHODS: The RmMAPRC protein sequence was analyzed with bioinformatics tools, and its structure was characterized by three-dimensional (3D) modeling and molecular docking. A semi-quantitative reverse transcription and polymerase chain reaction (sqRT-PCR) assessed the RmMAPRC gene presence and relative expression in tick organs and embryonic cells.
RESULTS: RmMAPRC relative expression in salivary glands, ovaries, and embryonic cells showed overexpression of 3%, 13%, and 24%, respectively. Bioinformatic analysis revealed that RmMAPRC corresponded to a Progesterone Receptor Membrane Component 1 (RmPGRMC1) of ~23.7 kDa, with an N-terminal transmembrane domain and a C-terminal Cytochrome b5-like heme/steroid binding domain. The docking results suggest that RmPGRMC1 could bind to progesterone (P4), some progestins, and P4 antagonists. The phylogenetic reconstruction showed that Rhipicephalus spp. MAPRC receptors were clustered in a clade that includes R. appendiculatus, R. sanguineus, and R. microplus (RmMAPRC), and mammals and helminths MAPRC receptors clustered in two separated clades away from ticks.
CONCLUSIONS: The presence of RmPGRMC1 highlights the importance of transregulation as a conserved adaptive mechanism that has succeeded for arthropod parasites, making it a target for tick control.