PDF(Pubmed)
Abstract：
UNASSIGNED: The integration of diagnostic methods holds promise for advancing the surveillance of malaria transmission in both endemic and non-endemic regions. Serological assays emerge as valuable tools to identify and delimit malaria transmission, serving as a complementary method to rapid diagnostic tests (RDT) and thick smear microscopy. Here, we evaluate the potential of antibodies directed against peptides encompassing the entire amino acid sequence of the PvMSP-1 Sal-I strain as viable serological biomarkers for P. vivax exposure.
UNASSIGNED: We screened peptides encompassing the complete amino acid sequence of the Plasmodium vivax Merozoite Surface Protein 1 (PvMSP-1) Sal-I strain as potential biomarkers for P. vivax exposure. Here, immunodominant peptides specifically recognized by antibodies from individuals infected with P. vivax were identified using the SPOT-synthesis technique followed by immunoblotting. Two 15-mer peptides were selected based on their higher and specific reactivity in immunoblotting assays. Subsequently, peptides p70 and p314 were synthesized in soluble form using SPPS (Solid Phase Peptide Synthesis) and tested by ELISA (IgG, and subclasses).
UNASSIGNED: This study unveils the presence of IgG antibodies against the peptide p314 in most P. vivax-infected individuals from the Brazilian Amazon region. In silico B-cell epitope prediction further supports the utilization of p314 as a potential biomarker for evaluating malaria transmission, strengthened by its amino acid sequence being part of a conserved block of PvMSP-1. Indeed, compared to patients infected with P. falciparum and uninfected individuals never exposed to malaria, P. vivax-infected patients have a notably higher recognition of p314 by IgG1 and IgG3.
UNASSIGNED: We screened peptides encompassing the complete amino acid sequence of the Plasmodium vivax Merozoite Surface Protein 1 (PvMSP-1) Sal-I strain as potential biomarkers for P. vivax exposure. Here, immunodominant peptides specifically recognized by antibodies from individuals infected with P. vivax were identified using the SPOT-synthesis technique followed by immunoblotting. Two 15-mer peptides were selected based on their higher and specific reactivity in immunoblotting assays. Subsequently, peptides p70 and p314 were synthesized in soluble form using SPPS (Solid Phase Peptide Synthesis) and tested by ELISA (IgG, and subclasses).
UNASSIGNED: This study unveils the presence of IgG antibodies against the peptide p314 in most P. vivax-infected individuals from the Brazilian Amazon region. In silico B-cell epitope prediction further supports the utilization of p314 as a potential biomarker for evaluating malaria transmission, strengthened by its amino acid sequence being part of a conserved block of PvMSP-1. Indeed, compared to patients infected with P. falciparum and uninfected individuals never exposed to malaria, P. vivax-infected patients have a notably higher recognition of p314 by IgG1 and IgG3.
摘要：
■诊断方法的整合有望在流行和非流行地区推进疟疾传播的监测。血清学检测是鉴别和界定疟疾传播的有价值的工具，作为快速诊断测试（RDT）和厚涂片显微镜的补充方法。这里,我们评估了针对包含PvMSP-1Sal-I菌株的整个氨基酸序列的肽的抗体作为间日疟原虫暴露的可行血清学生物标志物的潜力。
我们筛选了包含间日疟原虫孢子表面蛋白1(PvMSP-1)Sal-I菌株的完整氨基酸序列的肽作为间日疟原虫暴露的潜在生物标志物。这里,使用SPOT合成技术，然后进行免疫印迹，鉴定了由感染间日疟原虫的个体的抗体特异性识别的免疫显性肽。在免疫印迹测定中,基于它们的较高且特异的反应性来选择两个15聚体肽。随后，使用SPPS（固相肽合成）以可溶性形式合成肽p70和p314，并通过ELISA（IgG，和子类）。
■这项研究揭示了来自巴西亚马逊地区的大多数间日疟原虫感染个体中针对肽p314的IgG抗体的存在。计算机B细胞表位预测进一步支持利用p314作为评估疟疾传播的潜在生物标志物。由于其氨基酸序列是PvMSP-1保守区块的一部分而得到加强。的确,与感染恶性疟原虫的患者和从未接触过疟疾的未感染个体相比，间日疟原虫感染的患者通过IgG1和IgG3对p314的识别明显更高。
我们筛选了包含间日疟原虫孢子表面蛋白1(PvMSP-1)Sal-I菌株的完整氨基酸序列的肽作为间日疟原虫暴露的潜在生物标志物。这里,使用SPOT合成技术，然后进行免疫印迹，鉴定了由感染间日疟原虫的个体的抗体特异性识别的免疫显性肽。在免疫印迹测定中,基于它们的较高且特异的反应性来选择两个15聚体肽。随后，使用SPPS（固相肽合成）以可溶性形式合成肽p70和p314，并通过ELISA（IgG，和子类）。
■这项研究揭示了来自巴西亚马逊地区的大多数间日疟原虫感染个体中针对肽p314的IgG抗体的存在。计算机B细胞表位预测进一步支持利用p314作为评估疟疾传播的潜在生物标志物。由于其氨基酸序列是PvMSP-1保守区块的一部分而得到加强。的确,与感染恶性疟原虫的患者和从未接触过疟疾的未感染个体相比，间日疟原虫感染的患者通过IgG1和IgG3对p314的识别明显更高。
